Original article

A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan–induced gastroenterocolitis

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Abstract

Aim: To investigate the lipid membranes of rat enterocytes in chronic carrageenan-induced gastroenterocolitis accompanied by the activation of apoptotic processes.

Methods: Steady-state fluorescence spectroscopy: a study by fluorescent probes-by ortho-hydroxy derivatives of 2,5-diaryl-1,3oxazole. Activity of apoptosis signal-regulating kinase 1 and poly (ADP-ribose) polymerase in small intestinal homogenates, blood serum levels of matrix metalloproteinase-2 and caspase-3 and the level of DNA fragmentation in small intestinal homogenates were determined.

Results: Biochemical analysis revealed that an activation of apoptotic processes occurred in the intestinal epithelium of rats during chronic carrageenan-induced gastroenterocolitis. The fluorescence probes showed that activation of apoptotic processes in carrageenan-induced gastroenterocolitis was accompanied by changes in polar regions of rat enterocyte membranes, while no changes were revealed in more hydrophobic regions of the membranes.

Conclusion: The increase in hydration of membranes was attributed to the activation of the apoptosis of enterocytes. It has been shown that a fluorescent probe (2-(2′-hydroxyphenyl)-5-phenyl-1,3-oxazole) can be used for the detection of apoptosis of enterocytes.

Keywords: apoptosis, caspases, food additives, rats, inflammation

How to Cite:

Tkachenko, A., Marakushyn, D., Kalashnyk, I., Korniyenko, Y., Anatolii, O., Gorbach, T., Nakonechna, O., Posokhov, Y. & Tsygankov, A., (2018) “A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan–induced gastroenterocolitis”, Medicinski glasnik 15(2), 87-92. doi: https://doi.org/10.17392/946-18