Original article

A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan–induced gastroenterocolitis

Authors

• • Anton Tkachenko Anton Tkachenko ORCID profile. (opens in new tab)
  • Kharkiv National Medical University, Kharkiv, Department of Biochemistry, Ukraine
• • Dmytro Marakushyn
  • Kharkiv National Medical University, Kharkiv, Department of Physiology, Ukraine
• • Iuliia Kalashnyk
  • Kharkiv National Medical University, Kharkiv, Department of Otorhinolaryngology, Ukraine
• • Yevgen Korniyenko
  • V. N. Karazin Kharkiv National University, Kharkiv, Department of Human and Animal Physiology, Ukraine
• • Onishchenko Anatolii
  • Kharkiv National Medical University, Kharkiv, Department of Biochemistry, Ukraine
• • Tatyana Gorbach
  • Kharkiv National Medical University, Kharkiv , Department of Biochemistry, Ukraine
• • Oksana Nakonechna
  • Kharkiv National Medical University, Kharkiv , Department of Biochemistry, Ukraine
• • Yevgen Posokhov
  • The National Technical University “Kharkiv Polytechnic Institute”, Kharkiv, Department of Organic Chemistry, Biochemistry and Microbiology, Ukraine
• • Alexander Tsygankov
  • The National Technical University “Kharkiv Polytechnic Institute”, Kharkiv, Department of Organic Chemistry, Biochemistry and Microbiology, Ukraine

Abstract

Aim: To investigate the lipid membranes of rat enterocytes in chronic carrageenan-induced gastroenterocolitis accompanied by the activation of apoptotic processes.

Methods: Steady-state fluorescence spectroscopy: a study by fluorescent probes-by ortho-hydroxy derivatives of 2,5-diaryl-1,3oxazole. Activity of apoptosis signal-regulating kinase 1 and poly (ADP-ribose) polymerase in small intestinal homogenates, blood serum levels of matrix metalloproteinase-2 and caspase-3 and the level of DNA fragmentation in small intestinal homogenates were determined.

Results: Biochemical analysis revealed that an activation of apoptotic processes occurred in the intestinal epithelium of rats during chronic carrageenan-induced gastroenterocolitis. The fluorescence probes showed that activation of apoptotic processes in carrageenan-induced gastroenterocolitis was accompanied by changes in polar regions of rat enterocyte membranes, while no changes were revealed in more hydrophobic regions of the membranes.

Conclusion: The increase in hydration of membranes was attributed to the activation of the apoptosis of enterocytes. It has been shown that a fluorescent probe (2-(2′-hydroxyphenyl)-5-phenyl-1,3-oxazole) can be used for the detection of apoptosis of enterocytes.

Keywords: apoptosis, caspases, food additives, rats, inflammation

How to Cite: Tkachenko, A. , Marakushyn, D. , Kalashnyk, I. , Korniyenko, Y. , Anatolii, O. , Gorbach, T. , Nakonechna, O. , Posokhov, Y. & Tsygankov, A. (2018) “A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan–induced gastroenterocolitis”, Medicinski glasnik. 15(2). doi: https://doi.org/10.17392/946-18