Untargeted Urinary Metabolomic Profile of Isolated Chest Trauma Patients.
- Irshad Ahmad
(All India Institute of Medical Sciences, Rishikesh, Rishikesh, Trauma Surgery &Critical Care, India)
- Debabrata Sircar (Indian Institute of Technology Roorkee, Roorkee, Biosciences &Bioengineering, India)
- Madhur Uniyal (All India Institute of Medical Sciences Rishikesh, Rishikesh, Trauma Surgery & Critical Care, India)
- Mehvish Mukhtar (Government Medical College Anantnag, Anantnag, Pediatrics, India)
- Ajay Kumar (Rajendra Institute of Medical Sciences, Ranchi, General Surgery, India)
- Bhaskar Sarkar (All India Institute of Medical Sciences Rishikesh, Rishikesh, Trauma Surgery &Critical Care, India)
- Nilesh Jagne
(All India Institute of Medical Sciences, Nagpur, Nagpur, Department of Trauma Surgery , India)
- Md Qamar Azam (All India Institute of Medical Sciences Rishikesh, Rishikesh, Trauma Surgery &Critical Care, India)
Abstract
Untargeted Urinary Metabolomic Profile of Isolated Chest Trauma Patients. ABSTRACT:
Aim: To explore the untargeted urinary metabolomic biomarkers in isolated chest injury patients.
Methods: It was a prospective study for 1 year period including the patients of isolated chest trauma as per inclusion criteria. Day 0, 3, and 7 urine samples from patients and day 0 sample from age matched healthy relatives were collected. Samples were prepared and gas chromatography mass spectrometry analysis was done using Agilent Technologies. Multivariate analyses were done using the 'Biomarker Analysis' module of the MetaboAnalyst 6.0 to identify potential biomarkers associated with chest injuries. To identify the promising biomarkers with high sensitivity and specificity, exploratory multivariate (receiver operating characteristic) ROC curve analysis was used.
Results: A total of 31 patients and 31 healthy controls were enrolled. A total of 101 metabolites were annotated. Based on ROC analyses, the top 15 potential markers (azelaic acid, glycerol, anthranilic acid, 2-palmitoylglycerol, 2-hydroxyhippuric acid, 4-hydroxybenzeneacetic acid, arachidic acid, 3-hydroxyhippuric acid, adenine, sucrose, butanedioic acid, 4-hydroxyphenyllactic acid, butanoic acid, 4-hydroxybenzoic acid and 2-hydroxybenzeneacetic acid) were identified. Selected frequencies of >0.6% led to the selection of 9 biomarkers. It was found 66.5% predictive accuracy of the most accurate 50-feature panel of model with the AUC of 0.708 with 95% confidence interval.
Conclusion: Nine metabolites (azelaic acid, glycerol, anthranilic acid, 2-palmitoylglycerol, 2-hydroxyhippuric acid, 4-hydroxybenzeneacetic acid, arachidic acid, 3-hydroxyhippuric acid and adenine) based on their selected frequencies of more than 0.6% can be considered as biomarkers in isolated chest trauma patients.
Keywords: Gas Chromatography-Mass Spectrometry, Injury Severity Score, Metabolism, Thoracic Injuries
How to Cite:
Ahmad, I., Sircar, D., Uniyal, M., Mukhtar, M., Kumar, A., Sarkar, B., Jagne, N. & Azam, M. Q., (2025) “Untargeted Urinary Metabolomic Profile of Isolated Chest Trauma Patients.”, Medicinski glasnik 22(2), 330-338. doi: https://doi.org/10.17392/1954-22-02
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