Original article

Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6

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Abstract

Aim: To investigate the role of hypoxia-preconditioned mesenchymal stem cells (H-MSCs) in preventing peritoneal adhesion by regulating IL-6 at days 6 and 12.

Methods: Twenty-four PAs rat model weighing 250 g to 300 g were randomly allocated into 4 groups: sham (Sh), control (C), H-MSCs treatment group at dose 1.5 x 106 (T1) and 3 x 106 (T2). To induce H-MSCs, all MSCs population were incubated under hypoxia state (5% O2), 5% CO2, and 37o C for 24 hours. Expression level of IL-6 was performed using ELISA. Morphological appearance of adhesion was observed by visualizing the existence of adhesion formation in intestinal.

Results: In this study we found that there was a trend of decrease of IL-6 level on day 6 following MSCs treatments. Interestingly, there was a significant decrease of IL-6 level on day 12 in all treatment groups. Also, no adhesion occurred in T2 group.

Conclusions: H-MSCs prevent PA development by suppressing the prolonged release of IL-6 at proliferation phase.

Keywords: abdominal pain, abdominal surgery, cellular, inflammatory cytokines

How to Cite:

Muhar, A. M., Putra, A., Ekasaputra, V. M., Darlan, D. M., Paramita, D. A. & Adeputra Nasution, I. P., (2021) “Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6”, Medicinski glasnik 18(2), 398-403. doi: https://doi.org/10.17392/1304-21