Original article

Clitorea ternatea flower extract induces platelet-derived growth factor (PDGF) and GPx gene overexpression in ultraviolet (UV) B irradiationinduced collagen loss

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Abstract

Aim: To determine the effect of Clitorea ternatea flower extract (CTFE) in the gel dosage form on the expression of GPx and platelet-derived growth factor (PDGF) in ultraviolet (UV) - B irradiation-induced collagen loss rat model.

Methods: This is experimental research with post-test control group design. Twenty healthy male Wistar rats were divided into four treatment groups: a sham group, UVB control group, two treatment groups with gel of CTFE 5% and gel of CTFE 10%, respectively. Each group was treated with UVB at 302 nm with a MED of 160 mJ/cm2 for 5 days, whereas the sham group did not receive UVB. In the treatment groups CTFE 5% and CTFE 10% gel were given on the 6th to the 14th day. On day 14 all treatment groups were terminated, and GPx and PDGF gene expression were analysed using qRT-PCR.

Results: In the group of gel of CTFE 10%, there was a significant increase in GPx gene expression (9.51±1.83) and PDGF (4.36±1.18) compared to the UVB control group which had GPx and PDGF gene expression of 4.90±1.64) and 0.032±0.01, respectively.

Conclusion: The administration of CTFE gel showed an increase of the expression of GPx and PDGF genes on UVB irradiationinduced collagen loss rat model.

Keywords: clitorea ternatea flower extract, collagen loss, GPx, PDGF

How to Cite:

Putri, R. S., Putra, A., Chodidjah, D., Darlan, D. M., Trisnadi, S., Thomas, S., Amalina, N. D. & Irawan, R. C., (2022) “Clitorea ternatea flower extract induces platelet-derived growth factor (PDGF) and GPx gene overexpression in ultraviolet (UV) B irradiationinduced collagen loss”, Medicinski glasnik 20(1), 15-21. doi: https://doi.org/10.17392/1530-22