Original article

Association of endothelial nitric oxide synthase gene (G894T) polymorphism and hypertension in diabetic Bataknese patients

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Abstract

Aim: To assess endothelial nitric oxide synthase (eNOS) gene (G894T) polymorphism and nitric oxide (NO) level in hypertensive diabetic Bataknese patients.

Methods: A hospital-based, case control study (hypertensive and normotensive diabetic patient) was conducted. Genotyping of eNOS gene (G894T) was done using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Nitric oxide was quantified by sandwich enzyme-linked immunosorbent assay (Sandwich ELISA).

Results: GT polymorphism and T allele were higher in the hypertensive diabetic patients, 37.5% (p=0.6) and 54% (p=0.39), respectively. Nitric oxide level tended to be lower in the hypertensive diabetic patients (88.87µmol/L) comparing to the normotensive (95.42 µmol/L (p=0.54), as well as GT and TT polymorphism type (p=0.75).

Conclusion: eNOS gene (G894T) polymorphism is not associated with NO level and hypertension in the diabetic Bataknese patients.

Keywords: diabetes mellitus, hypertension, nitric oxide, nitric oxide synthase type III, polymorphism

How to Cite:

Siregar, J., Ganie, R. A., Lindarto, D., Mutiara, E. & Munir, D., (2020) “Association of endothelial nitric oxide synthase gene (G894T) polymorphism and hypertension in diabetic Bataknese patients”, Medicinski glasnik 17(2), 316-320. doi: https://doi.org/10.17392/1088-20