,
Department of Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University , Semarang , Indonesia
Department of Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University , Semarang , Indonesia
Department of Pathology Anatomy, Medical Faculty, Sultan Agung Islamic University , Semarang , Indonesia
Stem Cell and Cancer Research Indonesia , Semarang , Indonesia
Department of Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University , Semarang , Indonesia
Department of Surgery, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
Department of Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University , Semarang , Indonesia
Department of Surgery, Faculty of Medicine, Sultan Agung Islamic University , Semarang , Indonesia
Department of Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University , Semarang , Indonesia
Stem Cell and Cancer Research Indonesia
Pharmaceutical Sciences Department, Faculty of Medicine, Universitas Negeri Semarang , Semarang , Indonesia
Aim To investigate the effects of exosome-derived human mesenchymal stem cells (EH-MSCs) on matrix metalloproteinase-1 (MMP)-1 and malondialdehyde (MDA) levels in ultraviolet-B (UVB)-irradiated Wistar rats.
Methods The study used a post-test-only control group design with randomized allocation. Thirty rats were exposed to UVB radiation (302 nm, 150 mJ/cm²) for 8 minutes, five times a week over two weeks. Five groups were established: a healthy control (G1), a negative control (G2), a positive control that received a 200 μL subcutaneous injection of hyaluronic acid (HA) (G3), a treatment group 1 that received a 200 μL subcutaneous injection of exosome-derived EH-MSCs (G4), and a treatment group 2 that received a 300 μL subcutaneous injection of EH-MSCs (G5). An ELISA was used to quantify the levels of MDA and MMP-1.
Results A significant reduction in the mean level of MDA in groups G4 (368.33 ± 59.67) and G5 (329.33 ± 82.06) was noted when compared to the negative control group G2 (686.58 ± 119.01) (p<0.05). Similarly, MMP-1 showed a significant decrease in mean levels for G4 (0.08 ± 0.04) and G5 (0.07 ± 0.04) compared to G2 (0.33 ± 0.06) (p<0.05).
Conclusion These findings suggest that EH-MSCs have potent antioxidant and anti-inflammatory properties, mitigating UVB-induced skin damage by reducing oxidative stress markers.
Data curation, J.A.; Formal Analysis, J.A. and N.D.A.; Methodology, J.A.; Conceptualization, A.P.; Supervision, A.P., D.H., E.S. and T.S.; Validation, A.P.; Project administration, S.T.; Writing – review & editing, S.T., E.S. and T.S.; Visualization, N.D.A.; Writing – original draft, N.D.A. All authors have read and agreed to the published version of the manuscript.
The datasets generated and analyzed during the current study are available from the corresponding author upon reasonable request.
No
This work is licensed under a Attribution-NonCommercial-NoDerivatives 4.0 International 
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