Aim To investigate the effect of direct epineural electrical stimulation of the nerve on the nature of reparative processes in the bone stump. Methods Three series of experiments were carried out with amputation of the thigh in the middle third and muscle plasty. In the 1st and 2nd experimental series a perineural catheter was brought to the stump of the sciatic nerve, through which mechanical irritation of the nerve was performed for 20 days daily for 20 minutes. In the 2nd series, an electrode was added to the nerve and epineural electrical stimulation was performed daily for 20 days. Animals of the 3rd series served as control. The observation periods were 1, 3, 6 months. Histological research method with filling vessels with ink-gelatin mixture was applied. Results In the 1st series, there was a sharp distortion of the reparative process, which consisted of a violation of microcirculation, changes in shape, resorption of the cortical diaphyseal plate, fractures, deformations. In most experiments of the 2nd series, organotypic stumps were formed with normalization of microcirculation. In the 3rd series, results of the formation of the stump were better than in the 1st, but worse than in the 2nd series. Conclusions Painful nerve irritation after amputation leads to a significant disturbance of microcirculation and reparative regeneration at the end of the bone stump with the development of pathological restructuring of bone tissue. Electrostimulation of the nerve improves microcirculation and reparative regeneration of the bone tissue.
MacEwan MR, Gamble P, Stephen M, Ray WZ. Therapeutic electrical stimulation of injured peripheral nerve tissue using implantable thin-film wireless nerve stimulators. J Neurosurg 2018;130:486–95.
2
Zhou P, He F, Liu B, Wei S. Nerve electrical stimulation enhances osseointegration of implants in the beagle. Sci Rep 2019;9;(4916).
3
Tamaki H, Yotani K, Ogita F, Hayao K, Kirimto H, Onishi H, et al. Low-frequency electrical stimulation of denervated skeletal muscle retards muscle and trabecular bone loss in aged rats. Int J Med Sci 2019;16:822–30.
4
Beeve AT, Shen I, Zhang X, Magee K, Yan Y, MacEwan MR, et al. Neuroskeletal effects of chronic bioelectric nerve stimulation in health and diabetes. Front Neurosci 2021;15;(632768).
5
Das SK, Dhoonmoon L, Chhabra S. Neuromuscular stimulation of the common peroneal nerve increases arterial and venous velocity in patients with venous leg ulcers. Int Wound J 2021;18:187–93.
6
Bosanquet DC, Ivins N, Jones N, Harding KG. Microcirculatory flux and pulsatility in arterial Leg ulcers is increased by intermittent neuromuscular electrostimulation of the common peroneal nerve. Ann Vasc Surg 2021;71:308–14.
7
Abdullaev AS. Neuritis of the lower alveolar nerve and its treatment. GIRJ 2022;10;(515).
8
Shevchuk B, YO B, HV S, S.V. About the rational and irrational types of healing of the end bone with different methods of amputation plastics. Likars’ka Sprava 2018;6:130–40.
9
Grjibovski AM, Ivanov SV, Gorbatova MA. Analysis of quantitative data in two independent samples using Statistica and SPSS software: parametric and non-parametric tests. Nauka i Zdravookhranenie 2016;2:5–28.
10
Grjibovski AM, Ivanov SV, Gorbatova MA. Descriptive statistics using Statistica and SPSS software. Nauka i Zdravookhranenie 2016;1:7–23.
11
Rauck RL, Cohen SP, Gilmore CA, North JM, Kapural L, Zang RH, et al. Treatment of post-amputation pain with peripheral nerve stimulation. Neuromodulation 2014;17:188–97.
12
Gilmore CA, Ilfeld BM, Rosenow JM, Li S, Desai MJ, Hunter CW, et al. Percutaneous 60-day peripheral nerve stimulation implant provides sustained relief of chronic pain following amputation: 12-month follow-up of a randomized, double-blind, placebo-controlled trial. Reg Anesth Pain Med 2020;45:44–51.
13
Gilmore C, Ilfeld B, Rosenow J, Li S, Desai M, Hunter C, et al. Percutaneous peripheral nerve stimulation for the treatment of chronic neuropathic postamputation pain: a multicenter, randomized, placebo-controlled trial. Reg Anesth Pain Med 2019;44:637–45.
14
Cohen SP, Gilmore CA, Rauck RL, Lester DD, Trainer RJ, Phan T, et al. Percutaneous peripheral nerve stimulation for the treatment of chronic pain following amputation. Military Medicine 2019;8:267–74.
15
Gilmore C, Ilfeld B, Rosenow J, Li S, Desai M, Hunter C, et al. Percutaneous peripheral nerve stimulation for the treatment of chronic neuropathic postamputation pain: a multicenter, randomized, placebo-controlled trial. Reg Anesth Pain Med 2019;44:637–45.
16
Shevchuk B, YO B, HV D, TV J, Y. Changes in the structural organization of bone after amputation. Pol Ann Med 2020;27:147–53.
17
Ilfeld BM, Ball ST, Gabriel RA, Sztain JF, Monahan AM, Abramson WB. A feasibility study of percutaneous peripheral nerve stimulation for the treatment of postoperative pain following total knee arthroplasty. Neuromodulation 2019;22:653–60.
18
Deer TR, Gilmore CA, Desai MJ, Li SC, DePalma MJ, Hopkins TJ. Percutaneous peripheral nerve stimulation of the medial branch nerves for the treatment of chronic axial back pain in patients after radiofrequency ablation. Pain Med 2021;22:548–60.
19
Bezsmertnyi YO, Shevchuk J, Y B, HV B, O.Y. Impact of post-amputation pain syndrome on the results of bone stump formation. Morphologia 2021;15:50–6.
20
Lin YC, Kao CH, Chen CC, Ke CJ, Yao CH, Chen YS. Time-course effect of electrical stimulation on nerve regeneration of diabetic rats. PLoS One 2015;10:e0116711.
21
Dumanian GA, Potter BK, Mioton LM, Ko JH, Cheesborough JE, Souza JM, et al. Targeted muscle reinnervation treats neuroma and phantom pain in major limb amputees: A randomized clinical trial. Ann Surg 2019;70:238–46.
22
Baumgartner R, Botta P. Amputation und Prothesenversorgung. 2008.
23
Buchheit T, Hsia HJ, Cooter M, Shortell C, Kent M, McDuffie M, et al. The impact of surgical amputation and valproic acid on pain and functional trajectory n.d.
24
Shevchuk B, YO B, GV D, TV J, Y. Peculiar features of regeneration at the end of bone filing after amputation of a limb. World of Medicine and Biology 2021;1:229–34.
25
Shevchuk B, YO J, Y B, HV D, T.V. Reamputation stumps below knee. Med Glas (Zenica 2021;18:479–86.
26
Buch NS, Qerama E, Brix Finnerup N, Nikolajsen L. Neuromas and postamputation pain. Pain 2020;161:147–55.
27
Allami M, Faraji E, Mohammadzadeh F, Soroush MR. Chronic musculoskeletal pain, phantom sensation, phantom and stump pain in veterans with unilateral below-knee amputation. Scand J Pain 2019;19:779–87.
28
Ahuja V, Thapa D, Ghai B. Strategies for prevention of lower limb post-amputation pain: A clinical narrative review. J Anaesthesiol Clin Pharmacol 2018;34:439–49.
29
Ahmed A, Bhatnagar S, Mishra S, Khurana D, Joshi S, Ahmad SM. Prevalence of phantom limb pain, stump pain, and phantom limb sensation among the amputated cancer patients in India: a prospective, observational study. Indian J Palliat Care 2017;23:24–35.
30
Bezsmertnyi YO, Shevchuk J, Y. The influence of post-amputation pain syndrome and intraosseous main vessels on the formation of limb bone stump. Orthopaedics, Traumatology and Prosthetics 2022;2:20–5.
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