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Review paper
Published: 01.12.2022. Biochemistry
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https://doi.org/10.17392/1541-23

The role of mesenchymal stem cells in allergic rhinitis and its relationship with IL-10, plasma cells and regulatory T cells

By
Delfitri Munir Orcid logo ,
Delfitri Munir

Faculty of Medicine, Departement of Doctoral Degree Program,

Faculty of Medicine, Departement of Otorhinolaryngology-Head and Neck Surgery,

Pusat Unggulan, Iptek (PUI) Tissue Engineering,

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Iqbal Pahlevi Nasution ,
Iqbal Pahlevi Nasution

Departement of Paediatrics, Faculty of Medicine,

Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

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Lia Restimulia ,
Lia Restimulia

Departement of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine,

Pusat Unggulan, Iptek (PUI) Tissue Engineering,

Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

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Agung Putra ,
Agung Putra
Contact Agung Putra

Stem Cell and Cancer Research (SCCR) Indonesia, Indonesia

Department of Pathology, Medical Faculty, Sultan Agung Islamic University (UNISSULA),

Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA),

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Nur Dina Amalina
Nur Dina Amalina

Stem Cell and Cancer Research (SCCR) Indonesia,

Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia

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Abstract

Aim
Allergic rhinitis (AR) is an IgE-mediated inflammation of the nose. Regulatory T cells (Tregs), plasma cells and inflammatory cytokines have shown to play a critical role in allergic airway inflammation. The aim of the study was to investigate the role of mesenchymal stem cells (MSCs) in generating Treg cells and plasma cells associated with regulating interlukin-10 (IL-10) in AR model.
Methods
Fifteen male Wistar rats (6 to 8 weeks old) were randomly divided into three groups (control group, sham group, and
MSCs treatment group). Ovalbumin (OVA) nasal challenge was conducted daily from day 15 to 21, and MSCs (1x106
) were administrated intraperitoneally to OVA-sensitized rats on day 21. Sneezing was observed from day 24 to 27. The rats were sacrificed on day 24 and day 27. The expression of Treg and plasma cells was analysed by flow cytometry assay. The level of IL-10 was analysed under ELISA assay.
Results
This study showed that the percentage of sneezing and rubbing times significantly decreased in MSCs treatment associated with the regulation of IL-10 level and plasma cell. This finding was aligned with the significant increase of Treg level.
Conclusion
MSCs administration regulates IL-10 and plasma cell-mediated immune and inflammatory responses while increasing Treg cell production. MSCs may be a promising therapeutic target for treating Treg-mediated allergic diseases.

Keywords:

allergic rhinitis,
mesenchymal stem cells,
regulatory T cells,
IL-10

References

1.
Prescott S, Allen KJ. Food allergy: Riding the second wave of the allergy epidemic. Pediatric Allergy and Immunology. 2011;22:155–60.
2.
Wesemann DR, Nagler CR. The microbiome, timing, and barrier function in the context of allergic disease. Immunity. 2016;44:728–38.
3.
Small P, Keith PK, Kim H. Allergic rhinitis. Allergy Asthma Clin Immunol. 2018;14(Suppl 2).
4.
Wheatley LM, Togias A. Clinical practice. Allergic rhinitis N Engl J Med. 2015;372:456–63.
5.
bin WS, YQ D, J R, BK X, Z L, ZZ T. Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model. BMC Immunol. 2014;15(1).
6.
Wang S, Gao X, Shen G, Wang W, Li J, Zhao J, et al. Interleukin-10 deficiency impairs regulatory T cell-derived neuropilin-1 functions and promotes Th1 and Th17 immunity. Sci Rep. 2016;14(6).
7.
Chaudhry A, Samstein RM, Treuting P, Liang Y, Pils MC, Heinrich JM, et al. Interleukin-10 signaling in regulatory T cells is required for suppression of Th17 cell-mediated inflammation. Immunity. 2011;34:566–78.
8.
Stephen JG, Tsai M. IgE and mast cells in allergic disease. Nat Med. 2012;18:693–704.
9.
Darlan DM, Munir D, Putra A, Jusuf NK. MSCs-released TGFβ1 generate CD4+CD25+Foxp3+ in T-reg cells of human SLE PBMC. J Formos Med Assoc. 2021;120:602–8.
10.
Calzada D, Baos S, Cremades-Jimeno L, Cárdaba B. Immunological mechanisms in allergic diseases and allergen tolerance: The role of Treg cells. J Immunol Res. 2018;2018(6012053).
11.
Boonpiyathad T, Sözener ZC, Akdis M, Akdis CA. The role of treg cell subsets in allergic disease. Asian Pac J Allergy Immunol. 2020;38:139–49.
12.
Akdis M, Akdis CA. Therapeutic manipulation of immune tolerance in allergic disease. Nat Rev Drug Discov. 2009;8:645–60.
13.
Restimulia L, Ilyas S, Munir D, Madiadipoera T, Farhat F, Sembiring RJ, et al. Rats’ umbilical-cord mesenchymal stem cells ameliorate mast cells and Hsp70 on ovalbumin-induced allergic rhinitis rats. Med Glas. 2022;19(1).
14.
Kustiyah AR, Putra A, Nasihun T, Ramasamy R. The normal ratio of Th17 and Th1 post-mesenchymal stem cells coculture with PBMCs of systemic lupus erythematosus patients. Open Access Maced J Med Sci. 2021;9:169–76.
15.
Sungkar T, Putra A, Lindarto D, Sembiring RJ. Intravenous umbilical cord-derived mesenchymal stem cells transplantation regulates hyaluronic acid and Interleukin-10 secretion producing low-grade liver fibrosis in experimental rat. Med Arch. 2020;74:177–82.
16.
Drawina P, Putra A, Nasihun T, Prajoko YW, Dirja BT, Amalina ND. Increased serial levels of platelet‐ derived growth factor using hypoxic mesenchymal stem cell‐conditioned medium to promote closure acceler‐ation in a full‐thickness wound. Indonesian J Biotech. 2022;27:36–42.
17.
Li H, Tian Y, Xie L, Liu X, Huang Z, Su W. Mesenchymal stem cells in allergic diseases: Current status. Allergol Int. 2020;69:35–45.
18.
Sun L, Sha J, Meng C, Zhu D. Mesenchymal stem cell-based therapy for allergic rhinitis. Stem Cells Int. 2020;2020(2367524).
19.
Zaretsky AG, Konradt C, Dépis F, Wing JB, Goenka R, Atria DG, et al. T Regulatory cells support plasma cell populations in the bone marrow. Cell Rep. 2017;18:1906–16.
20.
Palomares O, Martin-Fontecha M, Lauener R, TraidlHoffmann C, Cavkaytar O, Akdis M, et al. Regulatory T cells and immune regulation of allergic diseases: Roles of IL-10 and TGF-β. Genes Immun. 2014;15:511–20.
21.
Barnig C, Bezema T, Calder PC, Charloux A, Frossard N, Garssen J, et al. Activation of resolution pathways to prevent and fight chronic inflammation: lessons from asthma and inflammatory bowel disease. Front immunol. 2019;10(1699).
22.
Liang KL, Yu SJ, Huang WC, Yen HR. Luteolin attenuates allergic nasal inflammation via Inhibition of Interleukin-4 in an allergic rhinitis mouse model and peripheral blood from human subjects with allergicrhinitis. Front Pharmacol. 2020;11(291).
23.
Prajoko YW, Putra A, Dirja BT, Muhar AM, Amalina ND. The ameliorating effects of MSCs in controlling treg-mediated B-Cell depletion by Indoleamine 2, 3-dioxygenase Induction in PBMC of SLE patients. Open Access Maced J Med Sci. 2022;10:6–11.
24.
Restimulia L, Ilyas S, Munir D, Putra A, Madiadipoera T, Farhat F, et al. The CD4+CD25+FoxP3+ regulatory t cells regulated by MSCs suppress plasma cells in a mouse model of allergic rhinitis. Med Arch. 2021;75(156).
25.
Hamra NF, Putra A, Tjipta A, Amalina ND, Nasihun T. Hypoxia mesenchymal stem cells accelerate wound closure improvement by controlling α-smooth muscle actin expression in the full-thickness animal model. Open Access Maced J Med Sci. 2021;9:35–41.
26.
Jang TY, Jung AY, Kim YH. Hormetic Effect of Chronic Hypergravity in a Mouse Model of Allergic Asthma and Rhinitis. Sci Rep. 2016;6:1–9.
27.
Ryu G, Bae JS, Kim JH, Kim EH, Chung YJ, Mo JH. Sneezing and rubbing counts in allergic rhinitis mouse models are a reliable indicator of type 2 immune response. Clin Exp Otorhinolaryngol. 2020;13:308–11.
28.
Blanc K, LC D. Mesenchymal stromal cells and the innate immune response. Immunol Lett. 2015;168:140–6.
29.
Weiss ARR, Dahlke MH. Immunomodulation by mesenchymal stem cells (MSCs): mechanisms of action of living, apoptotic, and dead MSCs. Front Immunol. 2019;10(1191).
30.
Putra A, Ridwan FB, Putridewi AI, Kustiyah AR, Wirastuti K, Sadyah NA, et al. The role of tnf-α induced mscs on suppressive inflammation by increasing tgf-β and il-10. Open Access Maced J Med Sci. 2018;6:1779–83.
31.
D MD, D M, N KJ, A P, R I, I A. In vitro regulation of IL-6 and TGF-ß by mesenchymal stem cells in systemic lupus erythematosus patients. Med Glas. 2020;17:408–13.
32.
Mayasari YI, Subchan P, Putra A, Chodijah C, Hussana A, Sumarawati T, et al. Secretome hypoxia mesenchymal stem cells inhibited ultraviolet radiation by inhibiting Interleukin-6 through nuclear factor-kappa beta pathway in hyperpigmentation animal models. Open Access Maced J Med Sci. 2023;11:188–94.
33.
Kamali AN, Noorbakhsh SM, Hamedifar H, JadidiNiaragh F, Yazdani R, Bautista JM, et al. A role for Th1-like Th17 cells in the pathogenesis of inflammatory and autoimmune disorders. Mol Immunol. 2019;105:107–15.
34.
Daryanti E, Putra A, Sumarawati T, Amalina ND, Prasetio A, Sidiq HA. The comparison of normoxic and hypoxic mesenchymal stem cells in regulating platelet-derived growth factors and collagen serial levels in skin excision animal models. Open Access Maced J Med Sci. 2023;11:181–7.
35.
Veen W, B S, G Y, M W, S S, DG A, et al. IgG4 production is confined to human IL-10-producing regulatory B cells that suppress antigen-specific immune responses. J Allergy Clin Immunol. 2013;131:1204–12.
36.
Tabarkiewicz J, Pogoda K, Karczmarczyk A, Pozarowski P, Giannopoulos K. The role of IL-17 and Th17 lymphocytes in autoimmune diseases. Archivum Immunologiae et Therapiae Experimentalis. 2015;63:435–49.
37.
Zhu Q, Rui K, Wang S, Tian J. Advances of regulatory B cells in autoimmune diseases. Front Immunol. 2021;12(592914).
38.
Nguyen T, CH P, YJ F, DU S, SY K, HJ S, et al. Anti-allergic rhinitis activity of α-lipoic acid via balancing Th17/ Treg expression and enhancing Nrf2/HO-1 pathway signaling. Sci Rep. 2020;10(12528).
39.
Ryu G, Bae JS, Kim JH, Kim EH, Chung YJ, Mo JH. Sneezing and rubbing counts in allergic rhinitis mouse models are a reliable indicator of type 2 immune response. Clin Exp Otorhinolaryngol. 2020;13:308–11.
40.
Saunders SP, Ma EGM, Aranda CJ, Lafaille MA. Non-classical B Cell memory of allergic IgE responses. Front Immunol. 2019;10(715).
41.
Zhang H, Kong H, Zeng X, Guo L, Sun X, He S. Subsets of regulatory T cells and their roles in allergy. J Transl Med. 2014;12:1–11.
42.
Robinson DS, Larché M, Durham. Tregs and allergic disease. J Clin Investig. 2004;114:1389–97.

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