,
Medical Education Study Program, Faculty of Medicine, Muhammadiyah Malang University Indonesia
Department of Pathology; Faculty of Medicine, Muhammadiyah Malang University Indonesia
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University; Malang, East Java Indonesia
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University; Malang, East Java Indonesia
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University; Malang, East Java Indonesia
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University; Malang, East Java Indonesia
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University; Malang, East Java Indonesia
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University; Malang, East Java Indonesia
Aim To evaluate the essential role of Moringa-albumin (MA) formulation in the maintenance of complement regulatory proteins through CD55 and CD59 on erythrocyte (TER-119).
Methods Strepto-zotocin (145 mg/kg) was used to induce diabetes mice. In diabetes mellitus (DM) model mice were treated with MA formulation for 14 days at a D1 - 500 mg/kg (M) + 620 mg/kg BW (A), D2 - 1000 mg/kg (M) + 420 mg/kg (A), and D3 - 1500 mg/kg (M) + 200 mg/kg (A). On the 15th day, the mice were dissected for the isolation of their bone marrow by the flushing method. The analysis flow cytometry was performed to find out the TER-119, CD55, and CD59 expressions. The data were statistically analyzed with one-way ANOVA (ρ≤ 0.05) and Tukey test using SPSS version 16 for Windows.
Results The effect of MA administration in D3 group have a significant effect on increasing the profile of erythrocytes (TER-119), compared to DM (ρ≤0.05) group (STZ Injection-no treatment), as well as CD59 expressed by erythrocytes. MA administration in D1 group and D3 group significantly increased the profile of CD55 expressed by erythrocytes compared to DM (ρ≤0.05).
Conclusions MA formulation with D3 group (1500 mg/kg (M) + 200 mg/kg (A)) was able to maintain or control complement regula-tory proteins (CD55 and CD59) on red blood cells (erythro-cytes/TER-119) in DM.
This study received no external funding.
Authors retain copyright. This work is licensed under a Creative Commons Attribution 4.0 International License. 
The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.