Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga , Surabaya, East Java , Indonesia
Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga , Surabaya , Indonesia
Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga , Surabaya, East Java , Indonesia
Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga , Surabaya, East Java , Indonesia
Department of Anatomy, Histology, and Pharmacology , Faculty of Medicine , Universitas Airlangga , Surabaya, East Java , Indonesia
Department of Internal Medicin, University Medical Centre Groningen , Groningen , Netherlands
Aim
Adipocyte-derived mesenchymal stem cells (AMSCs) have recently been studied as a potential new approach for regeneration treatment of heart failure and ischemic myocardium. However, donor’s age is thought to affect the ability of AMSC cells to differentiate into cardiomyocytes. This study aimed to analyse the association between donor’s age with the expression of cardiomyocyte marker cardiac isoform of Troponin-T (cTnT) quantity on AMSCs.
Methods
Subcutaneous adipose tissue was taken from donors of 3 different age groups (30-40, 41-50, and >50 years old), and
then processed into AMSC cells by insert culture. AMSC cell phenotype was identified by assessing the quantity of mesenchymal markers expression (CD34, CD45, CD90, CD105) using indirect immunofluorescence technique. Afterwards, cTnT expression, a marker for cardiomyocyte differentiation from AMSC cells, was evaluated under the fluorescent microscope. The comparison was done between the 3 different age groups.
Results
The comparative test and post hoc analysis showed that there were differences in the expression of CD34, CD45, CD90,
CD105, and cTnT between the three age groups (all p<0.05). Furthermore, there were strong negative correlations between age and CD34, CD90, CD105 and cTnT (r= -0.844, -0.914, -0.899, and -0.738, respectively), while the correlation was positive between age and CD45 (r=0.899).
Conclusion
Expression quantity of mesenchymal markers and cardiomyocyte markers from AMSC cells were affected by the
donor’s age. The higher the donor age group, the lower the expression quantity of mesenchymal markers (except CD45) and cTnT.
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