Snakehead fish extract as an enhancer of vascular endothelial growth factor and nitric oxide levels in cerebral angiogenesis: an insight of stroke therapy
Aim To assess the effect of snakehead fish extract administration in angiogenesis focusing on the level of vascular endothelial growth factor (VEGF), nitric oxide (NO) and VEGF receptor 2 (R2) expression is ischemic stroke models. Methods An experimental study was conducted on 5 groups of ischemic stroke rats models: Group K-without carotid artery ligation, Group K+ with artery ligation, Group P1 with artery ligation and administration of 200 mg/day extract, Group P2 with artery ligation and 400 mg/day extract, and Group P3 with artery ligation and 800 mg/day extract. The VEGF expression and NO levels were assessed on day 3. Results Snakehead fish extract significantly increased VEGF levels along with increasing doses, in which the highest VEGF level was observed in P3 group (361.7±40.2; p<0.001). The NO level also increased along with an increasing dose of snakehead fish extract, in which the highest NO level was found in P3 group (59.43±0.88 μmol/gr; p<0.001). The VEGFR2 expression also increased significantly after snakehead extract administration along with increasing doses (p<0.001) in which administration of 800 mg extract yielded the highest VEGFR2 expression compared with lower doses (17.7 vs. 15.6; p<0.001) Conclusion Snakehead fish extract administration increased angiogenesis process marked by an increased level of VEGF, NO and VEGFR2 expression in ischemic stroke rat models.
Liu J, Wang Y, Akamatsu Y, Lee C, Stetler R, Lawton Y. Vascular remodeling after ischemic stroke: mechanism and therapeutic potentials. Prog Neuobiol. 2014;138–56.
2.
Das A, Kim Y, Youn S, Varadarajan S. Cysteine oxidation of Cu importer CTR1 promotes VEGF signaling and angiogenesis. Circulation. 2016;1.
3.
Simon M, Welsh G, C. Mechanism and regulation of endothelial VEGF receptor signalling. Nat Rev Mol Cell Biol. 2016;611–25.
4.
Martin A. Imaging of perfusion, angiogenesis and tissue elasticity after stroke. J Cerebr Blood F Met. 2012;1296–507.
5.
Yao R, Zhang L, Li W, L. Cornel iridoid glycoside promotes neurogenesis and angiogenesis and improves neurological function after focal cerebral ischemia in rats. Brain Res Bull. 2009;69–76.
6.
Talwar T, Vashanta M, Srivastava P. Role of vascular endothel growth factor and other growth factors in post stroke recovery. Ann Indian Acad Neurol. 2014;1–6.
7.
Sen C, Khanna S, Venojarvi M, Trikha P. Cooperinduced vascular endothelial growth factor expression and wound healing. Am J Physiol Heart Circ. 2002;1821–7.
8.
Xiao B, Wang S, Yang G, Sun X, Lin L, Cheng J. HIF 1α contributes to hypoxia adaptation of the naked mole rat. Oncotarget. 2017;109941–51.
9.
Zhang R, Wang L, Zhang L, Chen J, Zhu Z, Zhang Z. Nitric oxide enhances angiogenesis via the synthesis of Vascular Endothelial Growth Factor and cGMP after stroke in the rat. Circ Res. 2003;308–13.
10.
Carmeliet P, Collen. Molecular basis of angiogenesis. Role of VEGF and VE-Cadherin. Ann N Y Acad Sci. 2000;249–64.
11.
Sahid N, Hayati F. Snakehead consumption enhances wound healing? From tradition to modern clinical practice: a prospective randomized controlled trial. Evid Based Complement Altternat Med. 2018;3032790.
12.
Chen K, Pittman R, Popel A. Nitric oxide in the vasculature: where does it come from and where does it go? A quantitative perspective. Antioxid Redox Signal. 2008;1185–95.
13.
Chen J, Zacharek A, Zhang C, Jiang H, Li Y, Roberts C, et al. Endothelial nitric oxide synthase regulates brain-derived neurotrophic factor expression and neurogenesis after stroke in mice. J Neurosci. 2005;2366–75.
14.
Gurtner G, Werner S, Barrandon Y, Longaker M. Wound repair and regeneration. Nature. 2008;314–21.
15.
Majewska I, Gendaszewska-Darmach E. Proangiogenic activity of plant extracts in accelerating wound healing -a new face of old phytomedicines. Acta Biochim Pol. 2011;449–60.
16.
Chopp M, Li Y, Chen J, Zhang R, Zhang Z. Brain repair and recovery from stroke. Eur Neurol. 2008;2–5.
17.
Hoeben A, Landuyt B, Highley M, Wildiers H, Van Oosterom A, Bruijn D, et al. Vascular endothelial growth factor and angiogenesis. Pharmacol Rev. 2004;549–80.
18.
Notoadmojo S, Metodologi Penelitian Kesehatan. Research methodology on health sciences). 2012;
19.
Pizem J, Cor A. Detection of apoptosis cells in tumour paraffin section. Radiol Oncol. 2003;225–32.
20.
Soini Y, Paakko P, Lehtovp. Histopathological evaluation of apoptosis in cancer. Am J Pathol. 1997;1041–8.
21.
Norhalifah N, Rahmawanty D, Nurlely N. Uji efektivitas ekstrak air ikan haruan (Channa striata) asal Kalimantan selatan terhadap bleeding time dan clotting time secara in vivo (Efficacy of snakehead fish extract (Channa striata) from South Borneo on bleeding time and clotting time in vivo. Media Farmasi. 2016;237–49.
22.
Siswanto A, Dewi N, Hayatie L. Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing. J Dentomaxillofac Sci. 2016;89–94.
23.
Bederson J, Pitts L, Tsuji M, Nishimura M, Davis R, Bartkowski H. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination. Stroke. 1986;472–6.
24.
Gandin C, Widmann C, Lazdunski M, Heurteaux C. Mlc901 favors angiogenesis and associated recovery after ischemic stroke in mice. Cerebrovasc Dis. 2016;139–54.
25.
Indra M, Gasmara C. Metode UCAO (Unilateral cerebral artery occlusion) meningkatkan kadar MMP-9 jaringan otak pada model tikus stroke iskemik (The UCAO methods succesfully increased MMP-9 level in brain tissue of ischemic stroke rat models) [Indonesian]. Malang Neurology Journal. 2016;1–5.
26.
Sköld M, Gertten C, Sandbergnordqvist A, Mathiesen T, Holmin S. VEGF and VEGF receptor expression after experimental brain contusion in rat. J Neurotrauma. 2005;353–67.
27.
Greenberg D, Jin. Vascular endothelial growth factors (VEGF) and stroke. Cell Mol Life Sci. 2013;1753–61.
28.
Mustafa A, Sujuti H, Permatasari N, Widodo M. Determination of nutrient contents and amino acid composition of Pasuruan Channa striata extract. IEESE International Journal of Science and Technology. 2013;1–11.
29.
Pudjonarko D, Abidin Z. IOP Conference Series: Earth and Environmental Science. 2018;12–28.
30.
Rahayu P, Sulisttyaningrum M, Suhartono E, M. Potential effect of striatin (DLBS0333), a bioactive protein fraction isolated from Channa striata for wound treatment. Asian Pac J Trop Biomed. 2016;1001–7.
31.
Oentaryo G, Istiati I, Soesilawati P. Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in Wister rats. Dental Journal. 2016;125–32.
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