Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara, Universitas Sumatera Utara , Medan , Indonesia
Pusat Unggulan Tissue Engineering, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
Pusat Unggulan Tissue Engineering, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
,
Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia
Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia
Department of Pathological Anatomy, Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semerang , Indonesia
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semerang , Indonesia
Aim
To analyse the ability of mesenchymal stem cells (MSCs) to regulate interleukin 6 (IL-6) and transforming growth factor (TGF-β) expression in vitro under co-culture conditions in human systemic lupus erythematosus (SLE).
Method
This study used a post-test group design that used peripheral blood mononuclear cells (PBMCs) from SLE patients at Kariadi Hospital, Semarang, Indonesia, and MSCs from a human umbilical cord. The cells were divided into two groups. The control group of PBMCs was treated with a standard medium, and the treatment group was co-cultured with the MSCs at a 1:40 ratio. Following 24 h incubation, the levels of IL-6 and TGF-β released in the culture medium were measured using a specific ELISA assay.
Results
This study showed a significant decrease in IL-6 level (p< 0.05) and a significant increase in TGF-β level (p<0.001) following 24 h of co-culture incubation of human SLE PBMCs cells and MSCs.
Conclusion
The PBMCs-to-MSCs ratio of 1:40 can regulate the IL-6 and TGF-β levels in human SLE PBMCs.
This work is licensed under a Attribution-NonCommercial-NoDerivatives 4.0 International 
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