,
Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
Pusat Unggulan Tissue Engineering, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
Pusat Unggulan Tissue Engineering, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
Stem Cell and Cancer Research (SCCR), Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia
Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia
Department of Pathological Anatomy, Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia
Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia
Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia
Pharmacy Study Program, Faculty of Mathematics and Natural Science, Universitas Negeri Semarang , Semarang , Indonesia
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sumatera Utara, Medan , Medan , Indonesia
Aim
Mesenchymal stem cells (MSCs) have potent immunosuppressive properties to control systemic lupus erythematosus (SLE) disease by inhibiting indoleamine 2,3-dioxygenase (IDO), and increasing regulatory T cells (Treg) to control innate and adaptive immune cells. However, the interaction and mechanism regarding IDO and B cells in the co-culture of MSC and SLE peripheral blood mononuclear cell (PBMCs) remain unclear. This study aimed to investigate the effects of MSCs in controlling B cells through IDO expression in PBMC of SLE patients.
Methods
This study used a post-test control group design. MSCs were obtained from human umbilical cord blood and characterized according to their surface antigen expression and multilineage differentiation capacities. PBMCs isolated from SLE patients were divided into five groups: sham, control, and three treatment groups. The treatment groups were treated by co-culturing MSCs to PBMCs with a ratio of 1:10, 1:25, and 1:40 for 72 h incubation. The B cell levels were analysed by flow cytometry with cytometric bead array (CBA) and the IDO levels were determined by ELISA.
Results
The percentages of B cells decreased significantly in groups treated by dose-dependent MSCs, particularly in T1 and T2
groups. These findings were aligned with the significant decrease of the IDO level.
Conclusion
MSCs control B cells-mediated by a decrease of IDO in PBMC of SLE patients.
This work is licensed under a Attribution-NonCommercial-NoDerivatives 4.0 International 
6
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