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Review paper
Received: 09.08.2021. >> Accepted: 03.11.2021. >> Published: 01.02.2022. Biochemistry
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https://doi.org/10.17392/1421-21

Rats’ umbilical-cord mesenchymal stem cells ameliorate mast cells and Hsp70 on ovalbumin-induced allergic rhinitis rats

By
Lia Restimulia ,
Lia Restimulia

Departement of Doctoral Degree Program, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia

Departement of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia

Pusat Unggulan IPTEK Tissue Engineering, Universitas Sumatera Utara , Medan , Indonesia

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Syafruddin Ilyas ,
Syafruddin Ilyas

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara , Medan , Indonesia

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Delfitri Munir ,
Delfitri Munir

Departement of Doctoral Degree Program, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia

Pusat Unggulan IPTEK Tissue Engineering, Universitas Sumatera Utara , Medan , Indonesia

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Agung Putra Orcid logo ,
Agung Putra
Contact Agung Putra

Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia

Department of Pathology, Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia

Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia

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Teti Madiadipoera ,
Teti Madiadipoera

Departement of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Padjadjaran/Hasan Sadikin General Hospital , Bandung , Indonesia

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Farhat Farhat ,
Farhat Farhat

Departement of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia

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Rosita Juwita Sembiring ,
Rosita Juwita Sembiring

Departement of Doctoral Degree Program, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia

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Muhammad Ichwan ,
Muhammad Ichwan

Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara , Medan , Indonesia

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Nur Dina Amalina
Nur Dina Amalina

Stem Cell and Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA) , Semarang , Indonesia

Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang , Semarang , Indonesia

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Abstract

Aim
Allergic rhinitis (AR) is a heterogeneous condition that has been associated with inflammatory responses and is characterized by clinical typical symptoms of nasal itching, sneezing, watery discharge and congestion. Mesenchymal stem cells (MSCs) are multipotent stem cells that have the immunoregulatory ability by secreting various cytokines which potent as a promising therapeutic modality for allergic airway diseases, including AR. The aim of this study was to investigate the effect of rat UC-MSCs on the number of mast cells, the expression of Hsp70 indicated by the nasal symptoms allergic, particularly nasal rubbing in ovalbumininduced AR rats.
Methods
Fifteen male Wistar rats (6 to 8 weeks old) were randomly divided into three groups (control group, sham group, and
OVA+MSCs group). OVA nasal challenge was conducted daily from day 15 to 21, and UC-MSCs (1x106
) were administrated intraperitoneally to OVA-sensitized rats on day 21. Nasal rubbing was observed from day 22 to 28. The rats were sacrificed on day 22 and day 28. The nasal cavity tissues were prepared for histological observations.
Results
The administration of UC-MSCs could reduce the number of mast cells and the expression of Hsp70 leading to reduction of
nasal symptoms allergic, particularly nasal rubbing.
Conclusion
Based on this finding, MSCs present a promising immediate curative effect to the inflammatory reaction in AR rats.

Keywords:

allergic rhinitis,
Hsp70,
mast cells,
MSCs,
ovalbumin

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Vol 19, Issue 1, 2022
Revealing the decrease of indoleamine 2,3-dioxygenase as a major constituent for B cells survival post-mesenchymal stem cells co-cultured with peripheral blood mononuclear cell (PBMC) of systemic lupus erythematosus (SLE) patients Rats’ umbilical-cord mesenchymal stem cells ameliorate mast cells and Hsp70 on ovalbumin-induced allergic rhinitis rats The effect of the shape of a clip on the magnetic field during magnetic resonance imaging examinations Pregnancy outcomes of COVID-19 positive pregnant women at the Cantonal Hospital Zenica, Bosnia and Herzegovina Relationship between depression and quality of life after myocardial infarction See full issue

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