Departement of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Padjadjaran/Hasan Sadikin General Hospital,
Bandung, Indonesia
Aim Allergic rhinitis (AR) is a heterogeneous condition that has been associated with inflammatory responses and is characterized by clinical typical symptoms of nasal itching, sneezing, watery discharge and congestion. Mesenchymal stem cells (MSCs) are multipotent stem cells that have the immunoregulatory ability by secreting various cytokines which potent as a promising therapeutic modality for allergic airway diseases, including AR. The aim of this study was to investigate the effect of rat UC-MSCs on the number of mast cells, the expression of Hsp70 indicated by the nasal symptoms allergic, particularly nasal rubbing in ovalbumininduced AR rats. Methods Fifteen male Wistar rats (6 to 8 weeks old) were randomly divided into three groups (control group, sham group, and OVA+MSCs group). OVA nasal challenge was conducted daily from day 15 to 21, and UC-MSCs (1x106 ) were administrated intraperitoneally to OVA-sensitized rats on day 21. Nasal rubbing was observed from day 22 to 28. The rats were sacrificed on day 22 and day 28. The nasal cavity tissues were prepared for histological observations. Results The administration of UC-MSCs could reduce the number of mast cells and the expression of Hsp70 leading to reduction of nasal symptoms allergic, particularly nasal rubbing. Conclusion Based on this finding, MSCs present a promising immediate curative effect to the inflammatory reaction in AR rats.
Li C, Fu Y, Wang Y, Kong Y, Li M, Ma D, et al. Mesenchymal stromal cells ameliorate acute allergic rhinitis in rats. Cell Biochem Funct. 2017. p. 420–5.
2.
Eifan A, Durham S. Pathogenesis of rhinitis. Clin Exp Allergy. 2016. p. 1139–51.
3.
Tang H, Han X, Li T, Feng Y, Sun J. Protective effect of miR-138-5p inhibition modified human mesenchymal stem cell on ovalbumin-induced allergic rhinitis and asthma syndrome. J Cell Mol Med. 2021. p. 5038–49.
4.
Varshney J, Varshney H. Allergic rhinitis: An overview. Indian J Otolaryngol Head Neck Surg. 2015. p. 143–9.
5.
Lee D, Choi H, Oh JM, Lee J, Lee J, Lee H, et al. Urban particulate matter regulates tight junction proteins by inducing oxidative stress via the Akt signal pathway in human nasal epithelial cells. Toxicol Lett. 2020. p. 33–41.
6.
Min H, Kim K, Yoon J, Kim C, Cho H. T-helper 2 cytokine-induced heat shock protein 70 secretion and its potential association with allergic rhinitis. Int Forum Allergy Rhinol. 2017. p. 530–5.
7.
Sun L, Sha J, Meng C, Zhu D. Mesenchymal stem cell-based therapy for allergic rhinitis. Stem Cells Int. 2020. p. 2367524.
8.
Hinds D, Aggarwal B, Du X, Mulgirigama A, Asia S. Pacific survey of physicians on asthma and allergic rhinitis (ASPAIR): data from China. Chin Med J (Engl). 2019. p. 1264–71.
9.
Gao F, Chiu S, Motan D, Zhang Z, Chen L, Ji H, et al. Mesenchymal stem cells and immunomodulation: Current status and future prospects. Cell Death Dis. 2016.
10.
Li Y, Zhong Y, Xiao X, Li D, Zhou Z, Tian Y. IL-33/ST2 axis promotes the inflammatory response of nasal mucosal epithelial cells through inducing the ERK1/2 pathway. Innate Immun. 2020. p. 505–13.
11.
Schachtele S, Clouser C, Aho J. Methods to validate mesenchymal stem cell identity, potency, and quality. R&D Systems; 2018.
12.
Numakura S, Uozaki H, Kikuchi Y, Watabe S, Togashi A, Watanabe M. Mesenchymal stem cell marker expression in gastric cancer stroma. Anticancer Res. 2019. p. 387–93.
13.
Blanc L, Mougiakakos K, D. Multipotent mesenchymal stromal cells and the innate immune system. Nat Rev Immunol. 2012. p. 383–96.
14.
Yang S, Park M, Yoon I, Kim S, Hong S, Shin J, et al. Soluble mediators from mesenchymal stem cells suppress T cell proliferation by inducing IL-10. Exp Mol Med. 2009. p. 315–24.
15.
Fu X, Chen Y, Xie F, Dong P, Liu W, Cao Y, et al. Comparison of immunological characteristics of mesenchymal stem cells derived from human embryonic stem cells and bone marrow. 2015. p. 616–22.
16.
Nakanishi K, Sato Y, Mizutani Y, Ito M, Hirakawa A, Higashi Y. Rat umbilical cord blood cells attenuate hypoxic-ischemic brain injury in neonatal rats. Sci Rep. 2017. p. 1–14.
17.
Yoo S, Chang D, Lee H, Kim G, Park J, Ryu B, et al. Immune following suppression mesenchymal stem cell transplantation in the ischemic brain is mediated by TGF-β. Neurobiol Dis. 2013. p. 249–57.
18.
Nemeth K, Keane-Myers A, Metcalfe J, Gorham D, Bundoc J, Hodges V, et al. Bone marrow stromal cells use TGF-β to suppress allergic responses in a mouse model of ragweed-induced asthma. Proc Natl Acad Sci U S A. 2010. p. 5652–7.
19.
Trisnadi S, Muhar A, Putra A, Kustiyah A. Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition. Univerca Medica. 2020. p. 97–104.
20.
Mollazadeh H, Cicero A, Blesso C, Pirro M, Majeed M, Sahebkar A. Immune modulation by curcumin: The role of interleukin-10. Crit Rev Food Sci Nutr. 2019. p. 89–101.
21.
Li M, Wan Y, Sanjabi S, Robertson AK, Flavell R. Transforming growth factor-β regulation of immune responses. Annu Rev Immunol. 2006. p. 99–146.
22.
Yohannes E, Chang J, Tar M, Davies K, Chance M. Molecular targets for diabetes mellitus-associated erectile dysfunction. Mol Cell Proteomics. 2010. p. 565–78.
23.
Zhao N, Liu Y, Liang H, Jiang X. Bone marrow-derived mesenchymal stem cells reduce immune reaction in a mouse model of allergic rhinitis. Am J Transl Res. 2016. p. 5628–36.
24.
Novoselova E, Glushkova O, Cherenkov D, Parfenyuk S, Novoselova T, Lunin S, et al. Production of heat shock proteins, cytokines, and nitric oxide in toxic stress. Biochem Biokhimii ͡ a. 2006. p. 376–83.
25.
Yang K, Liao Z, Wu Y, Li M, Guo T, Lin J, et al. Curcumin and Glu-GNPs induce radiosensitivity against breast cancer stem-like cells. Biomed Res Int. 2020. p. 3189217.
26.
Yombo D, Mentink-Kane M, Wilson M, Wynn T, Madala S. Heat shock protein 70 is a positive regulator of airway inflammation and goblet cell hyperplasia in a mouse model of allergic airway inflammation. J Biol Chem. 2019. p. 15082–94.
27.
Hamra N, Putra A, Tjipta A, Amalina N, Nasihun T. Hypoxia mesenchymal stem cells accelerate wound closure improvement by controlling α-smooth muscle actin expression in the full-thickness animal model. Open Access Maced J Med Sci. 2021. p. 35–41.
28.
Krainer F, Glieder A. An updated view on horseradish peroxidases: recombinant production and biotechnological applications. Appl Microbiol Biotechnol. 2015. p. 1611–25.
29.
Germic N, Frangez Z, Yousefi S, Hu S. Regu-lation of the innate immune system by autophagy: monocytes, macrophages, dendritic cells and antigen presentation. Cell Death Differ. 2019. p. 715–27.
30.
Taylor A, Verhagen J, Blaser K, Akdis M, Akdis C. Mechanisms of immune suppression by interleukin-10 and transforming growth factor-β: The role of T regulatory cells. Immunology. 2006. p. 433–42.
31.
Park I, Kim J, Bae J, Kim D, Mo J. The supernatant of tonsil-derived mesenchymal stem cell has antiallergic effects in allergic rhinitis mouse model. Mediators Inflamm. 2020. p. 6982438.
32.
Ebrahim N, Mandour Y, Farid A, Nafie E, Mohamed A, Safwat M, et al. Adipose tissue-derived mesenchymal stem cell modulates the immune response of allergic rhinitis in a rat model. Int J Mol Sci. 2019. p. 1–21.
33.
Shin T. Anti-inflammatory mechanisms of human umbilical cord blood-derived mesenchymal stem cells on atopic dermatitis and rheumatoid arthritis. 2016.
34.
Darlan D, Munir D, Putra A, Jusuf N. MSCs-released TGFβ1 generate CD4+CD25+Foxp3+ in T-reg cells of human SLE PBMC. J Formos Med Assoc. 2020. p. 1–7.
35.
Rossignol J, Boyer C, Thinard R, Remy S, Dugast A, Dubayle D, et al. Mesenchymal stem cells induce a weak immune response in the rat striatum after allo or xenotransplantation. J Cell Mol Med. 2009. p. 2547–58.
36.
Moloney T, Hoban D, Barry F, Howard L, Dowd E. Kinetics of thermally induced heat shock protein 27 and 70 expression by bone marrow-derived mesenchymal stem cells. Protein Sci. 2012. p. 904–9.
The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.