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Review paper
Received: 03.04.2023. >> Accepted: 07.11.2023. >> Published: 06.11.2023. Biochemistry
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https://doi.org/10.17392/1608-23

Hypoxic mesenchymal stem cells (MSCS)-induced interleukin (IL)-10 alleviate systemic lupus erythematosus (SLE) infl ammation through inhibiting interferon (IFN)-gamma production

By
Yan Wisnu Prajoko Orcid logo ,
Yan Wisnu Prajoko
Contact Yan Wisnu Prajoko

Department of Surgery, Faculty of Medicine, Universitas Diponegoro , Semarang , Indonesia

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Agung Putra ,
Agung Putra

Stem Cell and Cancer Research , Semarang , Indonesia

Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung , Semarang , Indonesia

Department of Pathology Anatomy, Faculty of Medicine, Universitas Islam Sultan Agung , Semarang , Indonesia

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Ardi Prasetio ,
Ardi Prasetio

Stem Cell and Cancer Research , Semarang , Indonesia

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Nur Dina Amalina
Nur Dina Amalina

Stem Cell and Cancer Research , Semarang , Indonesia

Pharmaceutical Sciences Department, Faculty of Medicine, Universitas Negeri Semarang , Semarang , Indonesia

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Abstract

Aim
To determine the eff ect of hypoxic mesenchymal stem cells (MSCs) on the interleukin (IL)-10 and interferon (IFN)-gamma in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients.
Methods
This study used a post-test control group design. Hypoxic MSCs were isolated and characterized according to their surface marker expression and diff erentiation capacities. PBMCs isolated from SLE patients were divided into three groups: control and two treatment groups. The treatment groups were treated by co-culturing MSCs to PBMCs with a ratio of 1:10 (T1) and 1:1 (T2) for 48 h incubation. Furthermore, IFN-gamma and IL-10 levels were determined by cytometric bead array (CBA) fl ow cytometry.
Results
Hypoxic MSCs signifi cantly decreased the IFN-gamma levels and increased the IL-10 levels in dose-dependent manner
compared to the control group. The highest activity of hypoxic MSCs was noticed in T2 group.
Conclusion
Hypoxic MSCs- induced IL-10 are important in the control of anti-infl ammatory eff ect on SLE through inhibiting IFN-gamma. 

Keywords:

hypoxic MSC,
IFN-gamma,
IL-10,
infl ammation,
PBMC,
SLE

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