Aim To identify E. coli from chicken meat, establish their antibiotic resistance profiles and to confirm ESBL isolates with real time PCR, as well as to identify risk factors and farming practice associated with the antimicrobial resistance E. coli. Methods The study included 100 chicken skin samples collected randomly from retail supermarkets, butcheries and slaughterhouses. Disk susceptibility testing was performed using the Kirby-Bauer method. Detection of ESBL-producing isolates was performed with double disk synergy test. Molecular analysis of phenotypic ESBL-producing Escherichia coli strains was performed at 7500 real time PCR System. Molecular-genetic analysis included detection of CTX-M 1, 2, and 9 gene families and mutations in the TEM and SHV encoding extended spectrum β-lactamases. Results Prevalence of the phenotypic ESBL-producing E. coli isolates was 29%, and they exhibited remarkable sensitivity to carbapenems (100%) as well as to amikacin (93.10%). All ESBL-producing strains were multidrug resistant. Molecular analysis was performed as the final confirmation of the production of extended spectrum β-lactamases for 24 isolates out of 29 phenotypicaly ESBL-producing E. coli isolates. Conclusion It is important to pay attention to people's awareness of bacterial antimicrobial resistance in food chain, as well as to understand its effects on human health and the environment. Phenotypic and molecular analysis demonstrated the presence of ESBL-producing E. coli isolates from chicken skin samples.
Rahman S, Khan A, I. Incidence of ESBL-producing-Escherichia coli in poultry farm environment and retail poultry meat. Pak Vet J 2019:116–20.
2
Microbiology of food and animal feeding stuffs -Preparation of test samples, initial suspensions and decimal dilutions for microbiological tests -Part 1: General rules for the preparation of initial suspensions and decimal dilutions. BAS EN ISO 1999;(1).
3
Horizontal method for glucuronidase positive Escherichia coli n.d.
4
Performance Standards for Antimicrobial Susceptibility Testing 2017.
5
Institute for applied laboratory analysis LTD. All-tissue DNA-extraction kit. Genial Germany 2007:11.
6
Check-MDR ESBL PCR Check Points. User Manual. Check Point The Netherlands 1 2015;(3).
7
Ten threats to global health in 2019.
8
Eyi A, Arslan S. Prevalence of Escherichia coli in retail poultry meat, ground beef and beef. Med Weter 2012.
9
Davis G, Waits K, Nordstrom L, Grande H, Weaver B, Papp K, et al. Antibiotic-resistant Escherichia coli from retail poultry meat with different antibiotic use claims. BMC Microbiol 2018:174.
10
Projahn M, Pacholewicz E, Becker E, Correia-Carreira G, Bandick N, Kaesbohrer A. Reviewing interventions against Enterobacteriaceae in broiler processing: using old techniques for meeting the new challenges of ESBL E. coli? Biomed Res Int 2018:7309346.
11
Reich F, Atanassova V, Klein G. Extended-Spectrumβ-Lactamase-and AmpC producing enterobacteria in healthy broiler chickens. Germany Emerg Infect Dis 2013:1253–9.
12
Mishra M, Patel A, Behera N. Prevalence of multidrug resistant E.coli in the river Mahanadi of Sambalpur. Curr Res Microbiol Biotechnol 2013:239–44.
13
Thenmozhi S, Rajeswari P, Kumar S, Saipriyanga T, Kalpana V, M. Multi-drug resistant patterns of biofilm forming Aeromonas hydrophila from urine samples. Int J Pharm Sci Res 2014:2908–18.
14
Joseph A, Odimayo M, Olokoba L, Olokoba A, Popoola G. Multiple antibiotic resistance index of Escherichia coli isolates in a tertiary hospital in southwest Nigeria. Medical Journal of Zambia 2017:225–32.
15
Hussain A, Shaik S, Ranjan A, Suresh A, Sarker N, Semmler T, et al. Genomic and functional characterization of poultry Escherichia coli from India revealed diverse extended-spectrum β-lactamaseproducing lineages with shared virulence profiles. Front Microbiol 2019.
16
Pacholewicz E, Barus S, Swart A, Havelaar A. Influence of food handlers’ compliance with procedures of poultry carcasses contamination: a case study concerning evisceration in broiler slaughterhouses. Food Control 2016:367–78.
17
Ghyselinck J. Antimicrobial resistance in human and broiler chicken Escherichia coli isolates 2008.
Landers T, Cohen B, Wittum T, El L. A review of antibiotic use in food animals: perspective, policy and potential. Public Health Rep 2012:4–22.
20
Qumar S, Majid M, Kumar N, Tiwari S, Semmler T, Devi S, et al. Genome dynamics and molecular infection epidemiology of multidrug-resistant Helicobacter pullorum isolates obtained from broiler and free-range chickens in India. Appl Environ Microbiol 2017:2305–16.
21
Zhang J, Massow A, Stanley M, Papariella M, Chen X, Kraft B, et al. Contamination rates and antimicrobial resistance in Enterococcus spp., Escherichia coli, and Salmonella isolated from “no antibiotics added”-labeled chicken products. Foodborne Pathog Dis 2011:1147–52.
22
Brower C, Mandal S, Hayer S, Sran M, Zehra A, Patel S, et al. The prevalence of extended-spectrum Beta lactamase-producing multidrug-resistant Escherichia coli in poultry chickens and variation according to farming practices in Punjab. Environ Health Perspect 2017:1–10.
23
Singer R, Hofacre C. Potential impacts of antibiotic use in poultry production. Avian Dis 2006:161–72.
24
Authority E. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2015. EFSA J 2016:4634.
25
Van Den Bogaard A, London N, Driessen C, Stobberingh E. Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. J Antimicrob Chemother 2001:763–71.
26
Meletis G. Carbapenem resistance: overview of the problem and future perspectives. Therap Adv Infec Dis 2016:15–21.
27
White A, Hughes J. Critical importance of a One Health approach to antimicrobial resistance. Eco Health 2019:404–9.
28
Lazarus B, Dpaterson L, Mollinger J, Rogers B. Do human extraintestinal Escherichia coli infections resistant to expanded-spectrum cephalosporins originate from food producing animals? A systematic review. Clin Infect Dis 2015:439–52.
29
Vitas A, Naik D, Pérez-Etayo L, González D. Increased exposure to extended-spectrum -lactamase producing multidrug-resistant Enterobacteriaceae through the consumption of chicken and sushi products. Int J Food Microbiol 2018:80–6.
30
Huijbers P, Graat E, Haenen A, Van Santen M, Van Essen-Zandbergen A, Mevius D, et al. Extended-spectrum and AmpC β-lactamase-producing Escherichia coli in broilers and people living and/or working on broiler farms: prevalence, risk factors and molecular characteristics. J Antimicrob Chemother 2014:2669–75.
31
Akond M. Antibiotic Resistance of Escherichia coli isolated from poultry and poultry environment of Bangladesh. Am J Environ Sci 2009:47–52.
32
Snary E, Kelly L, Davison H, Teale C. Wooldridge M. Antimicrobial resistance: a microbial risk assessment perspective. J Antimicrob Chemother 2004:906–17.
33
Castanon J. History of the use of antibiotic as growth promoters in European poultry feeds. Poult Sci 2007:2466–71.
34
Rahman S, Ali T, Ali I, Khan N, Han B, Gao J. The growing genetic and functional diversity of extended spectrum beta-lactamases. BioMed Res Int 2018.
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