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Review paper

Clitorea ternatea flower extract induces platelet-derived growth factor (PDGF) and GPx gene overexpression in ultraviolet (UV) B irradiationinduced collagen loss

By
Rizka Sofyanti Putri ,
Rizka Sofyanti Putri

Postgraduate Biomedical Sciences Program, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia

Agung Putra Orcid logo ,
Agung Putra
Contact Agung Putra

Stem Cell and Cancer Research Indonesia, Indonesia

Department of Postgraduate Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia

Departement of Pathology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia

Dr Chodidjah ,
Dr Chodidjah

Department of Postgraduate Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia

Dewi Masyitah Darlan ,
Dewi Masyitah Darlan

Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

Pusat Unggulan Tissue Engineering, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia

Setyo Trisnadi ,
Setyo Trisnadi

Department of Postgraduate Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia

Siti Thomas ,
Siti Thomas

Department of Postgraduate Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia

Nur Dina Amalina ,
Nur Dina Amalina

Stem Cell and Cancer Research Indonesia, Indonesia

Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia

Rizky Candra Irawan
Rizky Candra Irawan

Stem Cell and Cancer Research Indonesia, Indonesia

Abstract

Aim
To determine the effect of Clitorea ternatea flower extract (CTFE) in the gel dosage form on the expression of GPx and platelet-derived growth factor (PDGF) in ultraviolet (UV) - B irradiation-induced collagen loss rat model.
Methods
This is experimental research with post-test control group design. Twenty healthy male Wistar rats were divided into
four treatment groups: a sham group, UVB control group, two treatment groups with gel of CTFE 5% and gel of CTFE 10%,
respectively. Each group was treated with UVB at 302 nm with a MED of 160 mJ/cm2 for 5 days, whereas the sham group did not receive UVB. In the treatment groups CTFE 5% and CTFE 10% gel were given on the 6th to the 14th day. On day 14 all treatment groups were terminated, and GPx and PDGF gene expression were analysed using qRT-PCR.
Results
In the group of gel of CTFE 10%, there was a significant increase in GPx gene expression (9.51±1.83) and PDGF
(4.36±1.18) compared to the UVB control group which had GPx and PDGF gene expression of 4.90±1.64) and 0.032±0.01, respectively.
Conclusion
The administration of CTFE gel showed an increase of the expression of GPx and PDGF genes on UVB irradiationinduced collagen loss rat model.

References

1.
Subedi L, Lee T, Wahedi H, Baek S, Kim S. Resveratrol-enriched rice attenuates UVB-ROS-induced skin aging via downregulation of inflammatory cascades. Oxid Med Cell Longev. 2017.
2.
Mandal J, Shiue C, Chen Y, Lee M, Yang H, Chang H, et al. PKCδ mediates mitochondrial ROS generation and oxidation of HSP60 to relieve RKIP inhibition on MAPK pathway for HCC progression. Free Radic Biol Med. 2021. p. 69–87.
3.
Lim J, Kim O, Lee J, Lee M, Kang N, Hwang J. Protective effect of the standardized green tea seed extract on UVB-induced skin photoaging in hairless mice. Nutr Res Pract. 2014. p. 398–403.
4.
Rong J, Shan C, Liu S, Zheng H, Liu C, Liu M, et al. Skin resistance to UVB-induced oxidative stress and hyperpigmentation by the topical use of Lactobacillus helveticus NS8-fermented milk supernatant. J Appl Microbiol. 2017. p. 511–23.
5.
Zasada M, Budzisz E. Retinoids: active molecules influencing skin structure formation in cosmetic and dermatological treatments. Postepy Dermatolog Alergol. 2019. p. 392–7.
6.
Hada M, Mondul A, Weinstein S, Albanes D. Serum retinol and risk of overall and site-specific cancer in the ATBC study. Am J Epidemiol. 2020. p. 532–42.
7.
Iamsaard S, Burawat J, Kanla P, Arun S, Sukhorum W, Sripanidkulchai B, et al. Antioxidant activity and protective effect of Clitoria ternatea flower extract on testicular damage induced by ketoconazole in rats. J Zhejiang Univ Sci B. 2014. p. 548–55.
8.
Vidana Gamage G, Lim Y, Choo W. Anthocyanins From Clitoria ternatea Flower: Biosynthesis, Extraction, Stability, Antioxidant Activity, and Applications. Front Plant Sci. p. 792303.
9.
Ungur R, Borda I, Codea R, Ciortea V, Năsui B, Muste S, et al. A flavonoid-rich extract of Sambucus nigra L. reduced lipid peroxidation in a rat experimental model of gentamicin nephrotoxicity. Materials. 2022. p. 772–9.
10.
Lei L, Chen Y, Ou L, Xu Y, Yu X. Aqueous root extract of Asparagus cochinchinensis (Lour.) Merr. Has antioxidant activity in D-galactose-induced aging mice. BMC Complement Altern Med. 2017. p. 1–7.
11.
Ighodaro O, Akinloye O. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine. 2018. p. 287–93.
12.
Leyva-López N, Gutierrez-Grijalva E, Perez A, D, Heredia B, J. Flavonoids as cytokine modulators: a possible therapy for inflammation-related diseases. Int J Mol Sci. 2016. p. 921–30.
13.
Seo J, Lee H, Ryoo S, Seo J, Min B, Lee J. Tangeretin, a citrus flavonoid, inhibits PGDF-BBinduced proliferation and migration of aortic smooth muscle cells by blocking AKT activation. Eur J Pharmacol. 2011. p. 56–64.
14.
Xu B, Wang X, Wu C, Zhu L, Chen O, Wang X. Flavonoid compound icariin enhances BMP-2 induced differentiation and signalling by targeting to connective tissue growth factor (CTGF) in SAMP6 osteoblasts. PLoS One. 2018.
15.
Thilavech T, Adisakwattana S, Channuwong P, Radarit K, Jantarapat K, Ngewlai K, et al. Clitoria ternatea flower extract attenuates postprandial lipemia and increases plasma antioxidant status responses to a high-fat meal challenge in overweight and obese participants. Biology. 2021. p. 975–82.
16.
Nugraha A, Rahmadhani D, Puspitaningrum M, Rizqianti Y, Kharisma V, Ernawati D. Molecular docking of anthocyanins and ternatin in Clitoria ternatea as coronavirus disease oral manifestation therapy. J Adv Pharm Technol Res. 2021. p. 362–7.
17.
Zakaria N, Okello E, Howes M, Birch-Machin M, Bowman A. In vitro protective effects of an aqueous extract of Clitoria ternatea L. flower against hydrogen peroxide-induced cytotoxicity and UVinduced mtDNA damage in human keratinocytes. Phytotherapy Research. 2018. p. 1064–72.
18.
Amalina N, Wahyuni S, Harjito. Cytotoxic effects of the synthesized Citrus aurantium peels extract nanoparticles against MDA-MB-231 breast cancer cells. J Phys Conf Ser. 2021.
19.
Suzery M, Cahyono B, Amalina N. Antiproliferative and apoptosis effect of hyptolide from Hyptis pectinata ( L .) Poit on human breast cancer cells. Journal of Applied Pharmaceutical Science. 2020. p. 1–6.
20.
Fernanda M, Andriani R, Estulenggani Z, Kusumo G. Identification and determination of total flavonoids in ethanol extract of old and young angsana leaves (Pterocarpus indicus Willd.) using visible spectrophotometry. Scitepress. 2019. p. 541–4.
21.
I, M. Comparative phyto-constituents analysis from the root bark and root core extractives of Cassia ferruginea (Schrad D. C) plant. Scholars Journal of Agriculture and Veterinary Sciences. 2016. p. 275–83.
22.
Sulasmi S, Saptasari E, Mawaddah M, K, Zulfia A, F. Tannin identification of 4 species pterydophyta from baluran national park. Journal of Physics. Conference Series. Institute of Physics Publishing; 2019.
23.
Adu J, Amengor C, Kabiri N, Orman E, Patamia S, Okrah B. Validation of a simple and robust Liebermann-Burchard colorimetric method for the assay of cholesterol in selected milk products in Ghana. Int J Food Sci. 2019.
24.
Pękal A, Pyrzynska K. Evaluation of aluminium complexation reaction for flavonoid content assay. Food Anal Methods. 2014. p. 1776–82.
25.
Wise L, Stuart G, Real N, Fleming S, Mercer A. VEGF receptor-2 activation mediated by VEGF-E limits scar tissue formation following cutaneous injury. Adv Wound Care. 2018. p. 283–97.
26.
Putra A, Alif I, Hamra N, Santosa O, Kustiyah A, Muhar A, et al. MSC-released TGF-β regulate α-SMA expression of myofibroblast during wound healing. J Stem Cells Regen Med. 2020. p. 73–9.
27.
Hao J, Lou P, Han Y, Zheng L, Lu J, Chen Z, et al. Ultraviolet-B irradiation increases antioxidant capacity of Pakchoi (Brassica rapa L.) by inducing flavonoid biosynthesis. Plants. 2022. p. 776.
28.
Polefka T, Meyer T, Agin P, Bianchini R. Effects of solar radiation on the skin. J Cosmet Dermatol. 2012. p. 134–43.
29.
Brunetti C, Ferdinando D, Fini M, Pollastri A, Tattini S, M. Flavonoids as antioxidants and developmental regulators: relative significance in plants and humans. Int J Mol Sci. 2013. p. 3540–55.
30.
Hasanuzzaman M, Bhuyan M, Zulfiqar F, Raza A, Mohsin S, Mujita M, et al. Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antioxidants. 2020. p. 1–52.
31.
Bajpai V, Baek K, Kang S. Antioxidant and free radical scavenging activities of taxoquinone, a diterpenoid isolated from Metasequoia glyptostroboides. S Afr J Bot. 2017. p. 93–8.
32.
Sungkar T, Putra A, Lindarto D, Sembiring R. Intravenous umbilical cord-derived mesenchymal stem cells transplantation regulates hyaluronic acid and interleukin-10 secretion producing low-grade liver fibrosis in experimental rat. Med Arch. 2020. p. 177–82.
33.
Baby B, Antony, Halabi A, W, Homedi A, Vijayan Z, et al. Structural insights into the polypharmacological activity of quercetin on serine/threonine kinases. Drug Des Devel Ther. 2016. p. 3109.
34.
Addison R, Weatherhead S, Pawitri A, Smith G, Rider A, Grantham H, et al. Therapeutic wavelengths of ultraviolet B radiation activate apoptotic, circadian rhythm, redox signalling and key canonical pathways in psoriatic epidermis. Redox Biol. 2021. p. 101924.
35.
Karamichos D. Regulation of corneal fibroblast morphology and collagen. Journal Tissue Viability. 2009. p. 1–15.
36.
Al-Qattan M, Abd-Elwahed M, Hawary K, Arafah M, Shier M. Myofibroblast expression in skin wounds is enhanced by collagen III suppression. Biomed Res Int. 2015.
37.
Lin P, Chang H, Yeh C, Chang M, Chan C, Kuo H, et al. Transforming growth factor beta 1 increases collagen content, and stimulates procollagen I and tissue inhibitor of metalloproteinase-1 production of dental pulp cells: Role of MEK/ERK and activin receptorlike kinase-5/Smad signaling. J Formos Med Assoc. 2017. p. 351–8.
38.
Hamra N, Putra A, Tjipta A, Amalina N, Nasihun T. Hypoxia mesenchymal stem cells accelerate wound closure improvement by controlling α-smooth muscle actin expression in the full-thickness animal model. Open Access Maced J Med Sci. 2021. p. 35–41.
39.
Drawina P, Putra A, Nasihun T, Prajoko Y, Dirja B, Amalina N. Increased serial levels of plateletderived growth factor using hypoxic mesenchymal stem cell-conditioned medium to promote closure acceler-ation in a full-thickness wound. Indones J Biotechnol. 2022. p. 36–42.
40.
Luo Y, Ren Z, Du B, Xing S, Huang S, Li Y, et al. Structure identification of viceninii extracted from Dendrobium officinale and the reversal of TGF-β1-induced epithelial-mesenchymal transition in lung adenocarcinoma cells through TGF-β/Smad and PI3K/Akt/mTOR signaling pathways. Molecules. 2019. p. 144–52.
41.
Putra A, Rosdiana I, Darlan D, Alif I, Hayuningtyas F, Wijaya I, et al. Intravenous administration is the best route of mesenchymal stem cells migration in improving liver function enzyme of acute liver failure. Folia Med. 2020. p. 52–8.
42.
Cha B, Shi W, Yonezawa T, Teruya T, Nagai K, Woo J. An inhibitory effect of chrysoeriol on plateletderived growth factor (PDGF)-induced proliferation and PDGF receptor signaling in human aortic smooth muscle cells. J Pharmacol Sci. 2009. p. 105–10.
43.
Amalina N, Suzery M, Cahyono B. Cytotoxic activity of Hyptis pectinate extracts on MCF-7 human breast cancer cells. Indonesian Journal of Cancer Chemoprevention. 2020. p. 1–6.
44.
Masyithah Darlan D, Munir D, Jusuf K, Putra N, Ikhsan A, Alif R, et al. Med Glas. Zenica); 2020. p. 408–13.
45.
Restimulia L, Ilyas S, Munir D, Putra A, Madiadipoera T, Farhat F, et al. The CD4+ CD25+ FoxP3+ regulatory T cells regulated by MSCs suppress plasma cells in a mouse model of allergic rhinitis. Med Arch. 2021. p. 256.

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