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Review paper
Received: 20.01.2018. >> Accepted: 10.04.2018. >> Published: 25.01.2018. Biochemistry
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https://doi.org/10.17392/946-18

A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan–induced gastroenterocolitis

By
Anton Tkachenko Orcid logo ,
Anton Tkachenko
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Department of Biochemistry, Kharkiv National Medical University , Kharkiv , Ukraine

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Dmytro Marakushyn ,
Dmytro Marakushyn

Department of Physiology, Kharkiv National Medical University , Kharkiv , Ukraine

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Iuliia Kalashnyk ,
Iuliia Kalashnyk

Department of Otorhinolaryngology, Kharkiv National Medical University , Kharkiv , Ukraine

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Yevgen Korniyenko ,
Yevgen Korniyenko

Department of Human and Animal Physiology, V. N. Karazin Kharkiv National University , Kharkiv , Ukraine

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Onishchenko Anatolii ,
Onishchenko Anatolii

Department of Biochemistry, Kharkiv National Medical University , Kharkiv , Ukraine

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Tatyana Gorbach ,
Tatyana Gorbach

Department of Biochemistry, Kharkiv National Medical University , Kharkiv , Ukraine

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Oksana Nakonechna ,
Oksana Nakonechna

Department of Biochemistry, Kharkiv National Medical University , Kharkiv , Ukraine

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Yevgen Posokhov ,
Yevgen Posokhov

Department of Organic Chemistry, Biochemistry and Microbiology, The National Technical University “Kharkiv Polytechnic Institute” , Kharkiv , Ukraine

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Alexander Tsygankov
Alexander Tsygankov

Department of Organic Chemistry, Biochemistry and Microbiology, The National Technical University “Kharkiv Polytechnic Institute” , Kharkiv , Ukraine

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Abstract

Aim
 To investigate the lipid membranes of rat enterocytes in chronic carrageenan-induced gastroenterocolitis accompanied by the activation of apoptotic processes.
Methods
 Steady-state fluorescence spectroscopy: a study by fluorescent probes-by ortho-hydroxy derivatives of 2,5-diaryl-1,3oxazole. Activity of apoptosis signal-regulating kinase 1 and poly (ADP-ribose) polymerase in small intestinal homogenates, blood serum levels of matrix metalloproteinase-2 and caspase-3 and the level of DNA fragmentation in small intestinal homogenates were determined.
Results
 Biochemical analysis revealed that an activation of apoptotic processes occurred in the intestinal epithelium of rats during chronic carrageenan-induced gastroenterocolitis. The fluorescence probes showed that activation of apoptotic processes in carrageenan-induced gastroenterocolitis was accompanied by changes in polar regions of rat enterocyte membranes, while no changes were revealed in more hydrophobic regions of the membranes.
Conclusion
 The increase in hydration of membranes was attributed to the activation of the apoptosis of enterocytes. It has been shown that a fluorescent probe (2-(2′-hydroxyphenyl)-5-phenyl-1,3-oxazole) can be used for the detection of apoptosis of enterocytes.

Keywords:

apoptosis,
caspases,
food additives,
rats,
inflammation

References

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Vol 15, Issue 2, 2018
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