Aim To determine the effect of roselle (Hibiscus sabdariffa) petals in dextran sodium sulfate (DSS)-induced colitis model by measuring pro-inflammatory cytokines expressions (IL-6, and TNF-α), anti-inflammatory cytokine expression (IL-10) and histological colitis inflammation score (HCIS). Methods This study was conducted in two phases. For the first phase, five DSS-induced colitis mice were sacrificed (group 1) and compared with six healthy mice (group 2) after five-cycle induction (70 days). For the second phase, roselle-treated DSS-induced colitis mice were sacrificed on day 7, 14, 21, and 28 after induction and compared with mesalazine-treated DSS-induced colitis mice. Expressions of IL-6, TNF-α, and IL-10 were determined by immunohistochemistry and HCIS were assessed by two experienced pathologists. Results The expressions of IL-6, TNF-α, and IL-10, and HCIS in DSS-induced colitis mice were increased compared with control. The expressions of IL-6, TNF-α were significantly higher in roselle-treated group on day 7 and 14, but not on day 21 and 28, whereas, the expression of IL-10 was significantly lower only on day 7 compared with mesalazine-treated group. The inflammation was higher in roselle-treated group assessed by using HCIS. Compared to day 0, the reduction of HCIS was significant in roselle-treated and mesalazine-treated groups. Conclusion Roselle flower petal can attenuate the inflammation in DSS-induced colitis in mice. The extract of roselle can be given as an adjuvant therapy to the first-line therapy to enhance anti-inflammatory effect by increasing expression of anti-inflammatory cytokines and decreasing pro-inflammatory cytokines.
Anokwuru C, Esiaba I, Ajbaye O, Adesuyi A. Polyphenolic content and antioxidant activity of hibiscus sabdariffa calyx. Res J Med Plant 2011:557–66.
2
Ham M, Moss A. Mesalamine in the treatment and maintenance of remission of ulcerative colitis. Expert Rev Clin Pharmacol 2012:113–23.
3
Fakeye T. Toxicity and immunomodulatory activity of fractions of Hibiscus sabdariffa Linn (family Malvaceae) in animal models. Afr J Tradit Complem 2008:394–8.
4
Poritz L, Garver K, Green C, Fitzpatrick L, Ruggiero F, Koltun W. Loss of the tight junction protein ZO-1 in dextran sulfate sodium induced colitis. J Surg Res 2007:12–9.
5
Yuan B, Zhou S, Lu Y, Liu J, Wan J, Wang H, et al. Changes in the expression and distribution of claudins, increased epithelial apoptosis, and a mannan-binding lectin-associated immune response lead to barrier dysfunction in dextran sodium sulfate-induced rat colitis. Gut Liver 2015:734–40.
6
Eichele D, Kharbanda K. Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis. World J Gastroenterol 2017:6016–29.
7
Strober W, Fuss I, Mannon P. The fundamental basis of inflammatory bowel disease. J Clin Invest 2007:514–21.
8
Jammal M, Da Silva A, A, Filho M, De Castro Côbo A, Adad E, et al. Immunohistochemical staining of tumor necrosis factor-α and interleukin-10 in benign and malignant ovarian neoplasms. Oncol Lett 2015:979–83.
9
Laroui H, Ingersoll S, Liu H, Baker M, Ayyadurai S, Charania M, et al. Dextran sodium sulfate (DSS) induces colitis in mice by forming nano-lipocomplexes with medium-chain-length fatty acids in the colon. PLoS One 2012:32084.
10
Laurence D, Bacharach A. Evaluation of drug activities: pharmacometrics 1964.
11
Xu Q, Katakura Y, Yamashita M, Fang S, Tamura T, Matsumoto S, et al. IL-10 augments antibody production in in vitro immunized lymphocytes by inducing a Th2-type response and B cell maturation. Biosci Biotech Bioch 2004:2279–84.
12
Fakeye T, Pal A, Khanuja S. Anxiolytic and sedative effects of extracts of Hibiscus sabdariffa Linn (family Malvaceae). Afr J Med Med Sci 2008:49–54.
13
Chang Y-C, Huang H-P, Hsu J-D, Yang S-F, Wang C-J. Hibiscus anthocyanins rich extract-induced apoptotic cell death in human promyelocytic leukemia cells. Toxicol Appl Pharm 2005:201–12.
14
Matsuoka K, Kobayashi T, Ueno F, Matsui T, Hirai F, Inoue N, et al. Evidence-based clinical practice guidelines for inflammatory bowel disease. J Gastroenterol 2018:305–53.
15
Ilodibia C, Achebe U, Arubalueze C, Ibeh E. A comparative study on morphology, mineral and vitamin compositions of two variants of Hibiscus sabdariffa (L.). Arch Agri Environ Sci 2019:50–6.
16
Da-Costa-Rocha I, Bonnlaender B, Sievers H, Pischel I, Heinrich M. Hibiscus sabdariffa L. -A phytochemical and pharmacological review. Food Chem 2014:424–43.
17
Zhang H, Wang L, Li C, Yu Y, Yi Y, Wang J, et al. Exosome-induced regulation in inflammatory bowel disease. Front Immunol 2019:1464.
18
Nielsen O, Seidelin J, Munck L, Rogler G. Use of biological molecules in the treatment of inflammatory bowel disease. J Intern Med 2011:15–28.
19
Williams C, Panaccione R, Ghosh S, Rioux K. Optimizing clinical use of mesalazine (5-aminosalicylic acid) in inflammatory bowel disease. Therap Adv Gastroenterol 2011:237–48.
20
Liu B, Tonkonogy S, Sartor R. Antigen-presenting cell production of IL-10 inhibits T-helper 1 and 17 cell responses and suppresses colitis in mice. Gastroenterology 2011:653–62.
21
Keubler L, Buettner M, Häger C, Bleich A. A multihit model: colitis lessons from the interleukin-10-deficient mouse. Inflamm Bowel Dis 2015:1967–75.
22
Barman S, Kayama H, Okuzaki D, Ogino T, Osawa H, Matsuno H, et al. Identification of a human intestinal myeloid cell subset that regulates gut homeostasis. Int Immunol 2016:533–45.
23
Strober W, Fuss I. Pro-inflammatory cytokines in the pathogenesis of IBD. Gastroenterology 2011:1756–67.
24
Műzes G, Molnár B, Tulassay Z, Sipos F. Changes of the cytokine profile in inflammatory bowel diseases. World J Gastroenterol 2012:5848–61.
25
Bamias G, Kaltsa G, Ladas S. Cytokines in the pathogenesis of ulcerative colitis. Discovery Medicine 2011:459–67.
26
Sun M, He C, Cong Y, Liu Z. Regulatory immune cells in regulation of intestinal inflammatory response to microbiota. Mucosal Immunol 2015:969–78.
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