,
Sultan Agung Islamic University , Semarang , Indonesia
,
Department of Postgraduate Biomedical Sciences, Medical Faculty, UNISSULA, Semarang, Indonesia.
,
Sultan Agung Islamic University , Semarang , Indonesia
,
Soegijapranata Catholic University , Semarang , Indonesia
,
Sultan Agung Islamic University , Semarang , Indonesia
,
Sultan Agung Islamic University , Semarang , Indonesia
,
Sultan Agung Islamic University , Semarang , Indonesia
,
Sultan Agung Islamic University , Semarang , Indonesia
,
Sultan Agung Islamic University , Semarang , Indonesia
,
Sultan Agung Islamic University , Semarang , Indonesia
Sultan Agung Islamic University , Semarang , Indonesia
Sultan Agung Islamic University , Semarang , Indonesia
Aim to investigate the potential of SG followed by injection of MSCs in type 2 diabetic rats with obesity in improving IR.
Methods This study used a pre and post-control group design with 24 rats divided into three groups: Control (C), SG, and SG + MSCs (SG+M). On day 10, the level of vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-α), C-reactive protein (CRP), triglyceride (TG), pancreatic beta cells, and homeostasis model assessment of IR (HOMA-IR) was evaluated using qRT-PCR, ELISA, and immunohistochemistry.
Results A significant decrease of TNF-α (1.179 pg/mL), CRP(209 pg/mL), and TG levels (82,83 15,02pg/mL) in all treatment groups on day 10, in which SG + M group showed optimum inhibition was found (p < 0.05). This result was in line with the optimum increase of VEGF (8,1500 ± 2,47397) and pancreatic beta cell(10,1783±0,47) in SG + M group (p < 0.05). Moreover, our study also revealed the optimum decrease of HOMA-IR in SG+M group on day 10 (49,8233 ± 1,07303). Conclusion A combination of SG and MSCs can optimally improve insulin resistance by inhibiting TNF-α, CRP, and TG level and upregulating VEGF and pancreatic beta cell in an obese T2DM rat model
Conceptualization, V.M.E.S., T.S., S.I., G.H.A., N.L.S., R.M. and W.L.; Formal Analysis, V.M.E.S.; Methodology, V.M.E.S. and F.R.M.; Supervision, V.M.E.S., A.P., T.S. and S.I.; Writing – original draft, V.M.E.S. and F.R.M.; Writing – review & editing, V.M.E.S., T.S., S.I. and F.R.M.; Validation, A.P. and I.A.; Visualization, M.N.M., F.R.M., G.H.A., N.L.S., R.M. and W.L.; Project administration, A.D.A., G.H.A., N.L.S., R.M. and W.L.; Data curation, I.A. All authors have read and agreed to the published version of the manuscript.
No specific funding was received for this study
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