Aim To evaluate, in vitro antimicrobial ability of two probiotic toothpastes (one containing Lactobacillus paracasei, other containing Lactobacillus acidophilus) and one toothpaste without probiotic separately, and in a combination with two different mouthrinses (one containing essential oils and the other containing hexitidine). Methods Antimicrobial susceptibility was checked by using the ditch method and Clinical Laboratory Standard Institute (CLSI) guidelines. Two different toothpastes with probiotic, toothpaste without probiotic and two different mouthrinses were tested against the following selected microorganisms: Candida albicans, Candida tropicalis, Enterococcus faecalis, Streptococcus salivarius and Staphylococcus aureus. Kruskal-Wallis test and MannWhitney U test were used for the statistical analysis (p≤ 0.05). Results Probiotic toothpastes had better inhibitory effect than toothpaste without probiotic in the case of Candida albicans (p=0.043) and Streptococcus salivarius (p=0.043). In all cases, toothpastes had stronger inhibition capacity than mouthrinses (p≤0.05). Conclusion Probiotic toothpastes, as a relatively new concept in the prevention of oral infectious diseases such as caries and periodontal disease, can contribute to the prevention of oral infectious diseases.
Pinheiro E, Gomes B, Ferraz C, Teixeira F, Zaia A, Souza-Filho F. Evaluation of rot canal microorganisms isolted from teeth with endodontic failure and their antimicrobial susceptibility. Oral Microbiol Immunol 2003:100–3.
2
Polonskaia M. Antibiotic substances in acidophil bacteria. Mikrobiologija 1952:303–10.
3
Simark-Mattsson C, Emilson C, Håkansson E, Jacobsson C, Roos K, Holm S. Lactobacillus-mediated interference of mutans streptococci in caries-free vs. caries-active subjects. Eur J Oral Sci 2007:308–14.
4
Hasslöf P, Hedberg M, Twetman S, Stecksén-Blicks C. Growth inhibition of oral mutans streptococci and candida by commercial probiotic lactobacilli-an in vitro study. BMC Oral Health 2010:10–8.
5
Clinical Laboratory Standard Institute Performance standards for antimicrobial disc susceptibility tests; approved standard 2006:2-A9.
6
Glavina D, Goršeta K, Skrinjarić I, Vranić D, Mehulić K, Kozul K. Effect of LGG yoghurt on Streptococcus mutans and Lactobacillus spp. salivary counts in children. Coll Antropol 2012:129–32.
7
Koray M, Ak G, Kurklu E, Issever H, Tanyeri H, Kulekci G, et al. Fluconazole and/or hexetidine for management of oral candidiasis associated with denture-induced stomatitis. Oral Dis 2005:309–13.
8
Addy M, Wade W. An approach to efficacy screening of mouthrinses: studies on a group offrench products (I). J Clin Periodontol 1995:718–22.
9
Ellepola A, Samaranayake L. Oral candidal infections and antimycotics. Crit Rev Oral Biol Med 2000:172–98.
10
Majstorovic M, Vranic D, Szirovicza L. Recent achievements in preventive dentistry by introducing a new probiotic toothpaste. Coll Antropol 2013:1307–12.
11
Peciuliene V, Balciuniene I, Eriksen H, Haapasalo M. Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population. J Endod 2000:593–5.
12
Antimikrobna učinkovitost zubnih pasti koje sadrže probiotike: in vitro procjena n.d.
13
Parčina I, Amižić1. n.d.
14
Zdravlja Splitsko-Dalmatinske Županije D. Sveučilišna bolnica Split, 4 Medicinski fakultet 2AD.
15
Za statističku analizu korišten je Kruskal-Wallisov test i Mann-Whitney U-test n.d.
16
Rezultati Obje testirane zubne paste s probiotikom pokazale su zadovoljavajuće antimikrobno djelovanje, kako pojedinačno, tako i u kombinaciji s tekućinama za ispiranje usta n.d.
17
Zaključak Zubne paste s probioticima mogu doprinijeti u prevenciji oralnih zaraznih bolesti. Ključne riječi: Lactobacillus acidophilus, Lactobacillus paracasei, probiotici, tekućine za ispiranje usta n.d.
18
Shimauchi H, Mayanagi G, Nakaya S, Minamibuchi M, Ito Y, Yamaki K, et al. Improvement of periodontal condition by probiotics with Lactobacillus salivarius WB21: a randomized, double-blind, placebo-controlled study. J Clin Periodontol 2008:897–905.
19
Page R. The etiology and pathogenesis of periodontitis. Compend Contin Educ Dent 2002:11–4.
20
Gamboa F, Estupinan M, Galindo A. Presence of Streptococcus mutans in saliva and its relationship with dental caries: antimicrobial susceptibility of the isolates. Universitas Scientiarum 2004:23–7.
21
Mager D, Fyvie X, Haffajee L, A. Socransky SS. Distribution of selected bacterial species on intraoral surfaces. J Clin Periodontol 2003:644–54.
22
De Vrese M, Schrezenmeir J. Probiotics, prebiotics, and synbiotics. Adv Biochem Eng Biotechnol 2008:1–66.
23
Badet C, Thebaud N. Ecology of Lactobacilli in the oral cavity: a review of literature. Open Microbiol J 2008:38–48.
24
Ishikawa H, Aiba Y, Nakanishi M, Ohhashi Y, Koga Y. Suppression of periodontal pathogenic bacteria in the saliva of humans by the administration of Lactobacillus salivarius TI 2711. J Jpn Soc Periodontol 2003:105–12.
25
Krasse P, Carlsson B, Dahl C, Paulsson A, Nilsson A, Sinkiewicz G. Decreased gum bleeding and reduced gingivitis by the probiotic Lactobacillus reuteri. Swed Dent J 2006:55–60.
26
Matsuoka T, Sugano N, Takigawa S, Takane M, Yoshimura N, Ito K, et al. Effect of oral Lactobacillus salivarius TI 2711 (LS1) administration on periodontopathic bacteria in subgingival plaque. J Jpn Soc Periodontol 2006:315–24.
27
Haffajee A, Socransky S. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000:78–111.
28
Mayanagi G, Kimura M, Nakaya S, Hirata H, Sakamoto M, Benno Y, et al. Probiotic effect of orally administered Lactobacillus salivarius WB 21containing tablets on periodontopathic bacteria: a double-blind, placebo-controlled, randomized clinical trial. J Clin Periodontol 2009:506–13.
29
Iniesta M, Herrera D, Montero E, Zurbriggen M, Matos A R, Marin M, Sánchez-Beltrán M, et al. Probiotic effect of orally admi-nistered Lactobacillus reuteri-containing tablets on subgingival and salivary microbiota in patiens with gingivitis. A randomized clinical trial. J Clin Periodontol 2012:736–44.
30
Staab B, Eick S, Knöfler G, Jentsch H. The influence of a probiotic milk drink on the development of gingivitis: a pilot study. J Clin Periodontol 2009:850–6.
31
Twetman S, Derawi B, Keller M, Ekstrand K, Yucel-Lindberg T, Stecksén-Blicks C. Short-term effect of chewing gums containing probiotic Lactobacillus reuteri on the levels of inflammatory mediators in gingival crevicular fluid. Acta Odontol Scand 2009:19–24.
32
Meurman J. Probiotics: do they have a role in oral medicine and dentistry? Eur J Oral Sci 2005:188–96.
33
Teughels W, Loozen G, Ouirynen M. Do probiotics offer opportunities to manipulate the periodontal oral microbiota? J Clin Periodontol 2011:159–77.
34
Cannon M. A review of probiotic therapy in preventive dental practice. Probiotics Antimicrob Proteins 2011:63–7.
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