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Review paper
Received: 01.08.2016. >> Accepted: 05.12.2016. >> Published: 01.12.2016. Biochemistry
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https://doi.org/10.17392/870-16

Antimicrobial efficacy of probiotic-containing toothpastes: an in vitro evaluation

By
Ivana Parčina Amižić ,
Ivana Parčina Amižić

Dental Practice, Health Center of Split, Dalmatia County , Split , Croatia

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Livia Cigić ,
Livia Cigić

Department of Oral Medicine and Periodontology, School of Medicine, University of Split , Split , Croatia

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Lidia Gavić ,
Lidia Gavić
Contact Lidia Gavić

Department of Oral Medicine and Periodontology, School of Medicine, University of Split , Split , Croatia

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Marina Radić ,
Marina Radić

Clinical Department of Microbiology and Parasitology, University Hospital Centre Split , Split , Croatia

School of Medicine, University of Split , Split , Croatia

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Dolores Biočina Lukenda ,
Dolores Biočina Lukenda

Department of Oral Medicine and Periodontology, School of Medicine, University of Split , Split , Croatia

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Marija Tonkić ,
Marija Tonkić

Clinical Department of Microbiology and Parasitology, University Hospital Centre Split , Split , Croatia

School of Medicine, University of Split , Split , Croatia

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Ivana Goić Barišić
Ivana Goić Barišić

School of Medicine, University of Split , Split , Croatia

Clinical Department of Microbiology and Parasitology, University Hospital Centre Split , Split , Croatia

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Abstract

Aim
 To evaluate, in vitro antimicrobial ability of two probiotic toothpastes (one containing Lactobacillus paracasei, other containing Lactobacillus acidophilus) and one toothpaste without probiotic separately, and in a combination with two different mouthrinses (one containing essential oils and the other containing hexitidine).
Methods
Antimicrobial susceptibility was checked by using the ditch method and Clinical Laboratory Standard Institute (CLSI)
guidelines. Two different toothpastes with probiotic, toothpaste without probiotic and two different mouthrinses were tested against the following selected microorganisms: Candida albicans, Candida tropicalis, Enterococcus faecalis, Streptococcus salivarius and Staphylococcus aureus. Kruskal-Wallis test and MannWhitney U test were used for the statistical analysis (p≤ 0.05).
Results
 Probiotic toothpastes had better inhibitory effect than toothpaste without probiotic in the case of Candida albicans
(p=0.043) and Streptococcus salivarius (p=0.043). In all cases, toothpastes had stronger inhibition capacity than mouthrinses
(p≤0.05).
Conclusion
 Probiotic toothpastes, as a relatively new concept in the prevention of oral infectious diseases such as caries and periodontal disease, can contribute to the prevention of oral infectious diseases. 

Keywords:

Lactobacillus acidophilus,
Lactobacillus paracasei,
mouthrinse,
probiotics

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