Use of tcdC gene sequencing to prevent misidentification of Clostridioides difficile ribotype 176 and 027
Aim To compare the sequences of the tcdC gene between Clostridioides difficile (C. difficile) strains identified as PCR ribotype 176 and the reference strain C. difficile PCR ribotype 027 and to evaluate the use of the Xpert C. difficile/Epi assay for their differentiation. Methods A total of 45 strains were grown from storage beads. DNA of su...
By Tatiana Havrilova, Elena Novakova, Vincent Lucansky, Andrea Stanclova, Martina Neuschlova