OPTIMIZATION OF ILLUMINA® NEXTERA™ XT LIBRARY PREPARATION FOR THE MITOCHONDRIAL GENOME SEQUENCING AND CONFIRMATORY SANGER SEQUENCING
Aim: Due to increasing use of mitochondrial DNA (mtDNA) sequencing in both forensic practice and clinical disease research, this study explores the optimization of the next-generation sequencing (NGS) method for whole mitochondrial genome analysis on the Illumina MiSeq platform. Methods: Initial attempts using pre-made commercial primers were unsuc...
By Nejira Handžić, Dino Pećar, Selma Durgut, Naida Mulahuseinović, Ivana Čeko, Adna Ašić, Mirza Izmirlija, Sabina Šegalo, Lana Salihefendić, Rijad Konjhodžić