Hypoxic mesenchymal stem cells (MSCS)-induced interleukin (IL)-10 alleviate systemic lupus erythematosus (SLE) infl ammation through inhibiting interferon (IFN)-gamma production

Yan Wisnu Prajoko; Agung Putra; Ardi Prasetio; Nur Amalina

doi:10.17392/1608-23

Original article

Hypoxic mesenchymal stem cells (MSCS)-induced interleukin (IL)-10 alleviate systemic lupus erythematosus (SLE) infl ammation through inhibiting interferon (IFN)-gamma production

Authors

- Yan Wisnu Prajoko Yan Wisnu Prajoko ORCID profile. (opens in new tab)
- Universitas Diponegoro, Semarang, Faculty of Medicine, Department of Surgery, Indonesia
- Agung Putra
- Stem Cell and Cancer Research, Semarang, Indonesia
- Ardi Prasetio
- Stem Cell and Cancer Research, Semarang, Indonesia
- Nur Amalina
- Stem Cell and Cancer Research, Semarang, Indonesia

Abstract

Aim: To determine the eff ect of hypoxic mesenchymal stem cells (MSCs) on the interleukin (IL)-10 and interferon (IFN)-gamma in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients.

Methods: This study used a post-test control group design. Hypoxic MSCs were isolated and characterized according to their surface marker expression and diff erentiation capacities. PBMCs isolated from SLE patients were divided into three groups: control and two treatment groups. The treatment groups were treated by co-culturing MSCs to PBMCs with a ratio of 1:10 (T1) and 1:1 (T2) for 48 h incubation. Furthermore, IFN-gamma and IL-10 levels were determined by cytometric bead array (CBA) fl ow cytometry.

Results: Hypoxic MSCs signifi cantly decreased the IFN-gamma levels and increased the IL-10 levels in dose-dependent manner compared to the control group. The highest activity of hypoxic MSCs was noticed in T2 group.

Conclusion: Hypoxic MSCs- induced IL-10 are important in the control of anti-infl ammatory eff ect on SLE through inhibiting IFN-gamma.

Keywords: hypoxic MSC, IFN-gamma, IL-10, infl ammation, PBMC, SLE

How to Cite: Prajoko, Y. W. , Putra, A. , Prasetio, A. & Amalina, N. (2023) “Hypoxic mesenchymal stem cells (MSCS)-induced interleukin (IL)-10 alleviate systemic lupus erythematosus (SLE) infl ammation through inhibiting interferon (IFN)-gamma production”, Medicinski glasnik. 21(1). doi: https://doi.org/10.17392/1608-23