Original article

An ethanol extract of Senduduk fruit (Melastoma malabathricum L) inhibits the expression of vascular endothelial growth factor and tumour necrosis factor alpha in HeLa cells

Authors

• • Deri Edianto Deri Edianto ORCID profile. (opens in new tab)
  • Universitas Sumatera Utara, Faculty of Medicine, Department of Obstetrics and Gynaecology, Indonesia
• • Aznan Lelo
  • Universitas Sumatera Utara, Faculty of Medicine, Department of Pharmacology and Therapeutics, Indonesia
• • Syafruddin Ilyas
  • Faculty of Mathematics and Natural Science, Universitas Sumatera Utara, Indonesia
• • Marline Nainggolan
  • Faculty of Pharmacy, Universitas Sumatera Utara, Indonesia

Abstract

Aim: Senduduk fruit (Melastoma malabathricum L) is native to Indomalaya and is believed to possess anticancer activity. This study investigated antiangiogenic and anti-inflammation effects of an ethanolic extract of Senduduk fruit (EESF) on HeLa cells.

Methods: Cytotoxicity was assayed in HeLa cell cultures exposed to a concentration series of 500-7.8 µg/mL of EESF. IC 50 was determined with a methylthiazol-tetrazolium (MTT) cell viability assay. Antiangiogenic and anti-inflammation activity was evaluated by an immunocytochemical assay of vascular endothelial growth factor (VEGF) and tumour necrosis factor alpha (TNF-?) expression in HeLa cells cultured with 1× or 2× IC 50 or as a control without EESF.

Results: IC 50 of the EESF was 956 µg/mL. The intensity of VEGF staining indicated moderate expression in HeLa cells in response to IC 50 , weak expression in response to 2×IC 50 , and strong expression in the absence of the EESF. While the intensity of TNF-? staining indicated moderate expression in HeLa cells in response to IC 50 and to the absence of EESF, and weak expression in response to 2× IC 50.

Conclusion: Senduduk fruit extract inhibited VEGF and TNF-? expression in HeLa cells in a concentration-dependent manner.

Keywords: ethanol extract, HeLa cell, Melastoma malabathricum L fruit, TNF-α, VEGF

How to Cite: Edianto, D. , Lelo, A. , Ilyas, S. & Nainggolan, M. (2020) “An ethanol extract of Senduduk fruit (Melastoma malabathricum L) inhibits the expression of vascular endothelial growth factor and tumour necrosis factor alpha in HeLa cells”, Medicinski glasnik. 17(2). doi: https://doi.org/10.17392/1182-20